Overview

  • Product name
    Anti-RFX3 antibody
    See all RFX3 primary antibodies
  • Description
    Mouse polyclonal to RFX3
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Dog, Cynomolgus monkey, Xenopus tropicalis
  • Immunogen

    Full length Human RFX3 (amino acids 1-749; NP_602304.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • RFX3 transfected 293T cell lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab168475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Acts as a transcription factor. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells.
  • Sequence similarities
    Belongs to the RFX family.
    Contains 1 H-T-H motif winged-type DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • bA32F11.1 antibody
    • DNA binding protein RFX3 antibody
    • Regulatory factor X 3 antibody
    • Regulatory factor X, 3 (influences HLA class II expression) antibody
    • RFX3 antibody
    • RFX3_HUMAN antibody
    • Transcription factor RFX3 antibody
    see all

Images

  • Western blot - Anti-RFX3 antibody (ab168475)
    Western blot - Anti-RFX3 antibody (ab168475)
    All lanes : Anti-RFX3 antibody (ab168475) at 1 µg/ml

    Lane 1 : RFX3 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Predicted band size: 84 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RFX3 antibody (ab168475)
    Immunocytochemistry/ Immunofluorescence - Anti-RFX3 antibody (ab168475)
    Immunofluorescent analysis of HeLa cells labeling RFX3 with ab168475 at 10 µg/ml.

References

ab168475 has not yet been referenced specifically in any publications.

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