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    rfx3-antibody-ab168475.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Helix-Turn-Helix
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Anti-RFX3 antibody (ab168475)

  • Datasheet
  • SDS
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Western blot - Anti-RFX3 antibody (ab168475)
  • Immunocytochemistry/ Immunofluorescence - Anti-RFX3 antibody (ab168475)

Key features and details

  • Mouse polyclonal to RFX3
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-RFX3 antibody
    See all RFX3 primary antibodies
  • Description

    Mouse polyclonal to RFX3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Dog, Cynomolgus monkey, Xenopus tropicalis
  • Immunogen

    Full length Human RFX3 (amino acids 1-749; NP_602304.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • RFX3 transfected 293T cell lysate; HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.4
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Helix-Turn-Helix
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab168475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa.
ICC/IF
Use a concentration of 10 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa.
ICC/IF
Use a concentration of 10 µg/ml.

Target

  • Function

    Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Acts as a transcription factor. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells.
  • Sequence similarities

    Belongs to the RFX family.
    Contains 1 H-T-H motif winged-type DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P48380 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 476339 Dog
    • Entrez Gene: 100051128 Horse
    • Entrez Gene: 5991 Human
    • Entrez Gene: 19726 Mouse
    • Entrez Gene: 100009410 Rabbit
    • Entrez Gene: 361746 Rat
    • Entrez Gene: 779608 Xenopus tropicalis
    • Omim: 601337 Human
    • SwissProt: Q4R3I8 Cynomolgus monkey
    • SwissProt: P48380 Human
    • SwissProt: P48381 Mouse
    • SwissProt: Q0V9K5 Xenopus tropicalis
    • Unigene: 136829 Human
    • Unigene: 313321 Mouse
    • Unigene: 23347 Rat
    see all
  • Alternative names

    • bA32F11.1 antibody
    • DNA binding protein RFX3 antibody
    • Regulatory factor X 3 antibody
    • Regulatory factor X, 3 (influences HLA class II expression) antibody
    • RFX3 antibody
    • RFX3_HUMAN antibody
    • Transcription factor RFX3 antibody
    see all

Images

  • Western blot - Anti-RFX3 antibody (ab168475)
    Western blot - Anti-RFX3 antibody (ab168475)
    All lanes : Anti-RFX3 antibody (ab168475) at 1 µg/ml

    Lane 1 : RFX3 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Predicted band size: 84 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RFX3 antibody (ab168475)
    Immunocytochemistry/ Immunofluorescence - Anti-RFX3 antibody (ab168475)
    Immunofluorescent analysis of HeLa cells labeling RFX3 with ab168475 at 10 µg/ml.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab168475? Please let us know so that we can cite the reference in this datasheet.

ab168475 has not yet been referenced specifically in any publications.

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