Anti-RFX3 antibody (ab168475)
Key features and details
- Mouse polyclonal to RFX3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RFX3 antibody
See all RFX3 primary antibodies -
Description
Mouse polyclonal to RFX3 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Dog, Cynomolgus monkey, Xenopus tropicalis
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Immunogen
Full length Human RFX3 (amino acids 1-749; NP_602304.1).
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Positive control
- RFX3 transfected 293T cell lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa. |
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ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Acts as a transcription factor. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells. -
Sequence similarities
Belongs to the RFX family.
Contains 1 H-T-H motif winged-type DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 476339 Dog
- Entrez Gene: 100051128 Horse
- Entrez Gene: 5991 Human
- Entrez Gene: 19726 Mouse
- Entrez Gene: 100009410 Rabbit
- Entrez Gene: 361746 Rat
- Entrez Gene: 779608 Xenopus tropicalis
- Omim: 601337 Human
see all -
Alternative names
- bA32F11.1 antibody
- DNA binding protein RFX3 antibody
- Regulatory factor X 3 antibody
see all
Images
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Western blot - Anti-RFX3 antibody (ab168475)All lanes : Anti-RFX3 antibody (ab168475) at 1 µg/ml
Lane 1 : RFX3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 84 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RFX3 antibody (ab168475)Immunofluorescent analysis of HeLa cells labeling RFX3 with ab168475 at 10 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab168475 has not yet been referenced specifically in any publications.