Product nameRFX5 Kit
Species reactivityHuman RFX5 from a variety of fibroblast and B-cell lysates.
Anti-RFX5 (N-terminal specific) may react with unknown minor bands at 85 kDa and 55 kDa.
Tested applicationsSuitable for: ELISA, IP, WBmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Storage buffer0.01M Na phosphate, 0.15M NaCl, pH7.4, 0.1% Na azide
Components 1 kit ab7542 - Anti-RFX5 Antibody aa320-494 1 x 100µg Anti-RFX5 Antibody C-Terminal 1 x 100µg ab7543 - Anti-RFX5 Antibody N-Terminal 1 x 100µg Goat Anti-Rabbit IgG (H+L) HRP conjugated 1 x 100µg
FunctionActivates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.
Involvement in diseaseDefects in RFX5 are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
Sequence similaritiesBelongs to the RFX family.
Contains 1 RFX-type winged-helix DNA-binding domain.
DomainThe N-terminus is required for dimer formation, association with RFXANK and RFXAP, assembly of the RFX complex, and for binding of this complex to its X box target site in the MHC-II promoter. The C-terminus mediates cooperative binding between the RFX complex and NF-Y.
- Information by UniProt
- DNA binding protein RFX 5
- DNA binding protein RFX5
- DNA-binding protein RFX5
Our Abpromise guarantee covers the use of ab7544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.
A minor band at 68 kDa may be detected which may represent an RFX5 breakdown product or unmodified RFX5
|EMSA||Use at an assay dependent concentration.|
ab7544 has not yet been referenced specifically in any publications.