Anti-RFXAP antibody [RFXAD55A] (ab51376)

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Western blot - Anti-RFXAP antibody [RFXAD55A] (ab51376)

    Key features and details

    • Mouse monoclonal [RFXAD55A] to RFXAP
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    Overview

    • Product name

      Anti-RFXAP antibody [RFXAD55A]
      See all RFXAP primary antibodies

    • Description

      Mouse monoclonal [RFXAD55A] to RFXAP

    • Host species

      Mouse

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Recombinant fragment, corresponding to internal sequence amino acids 50-200 of Human RFXAP

    • Positive control

      • HeLa whole cell lysate

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

    • Storage buffer

      pH: 7.40
      Preservative: 0.05% Sodium azide
      Constituents: 1% BSA, PBS
    • Concentration information loading...

    • Purity

      Protein G purified

    • Purification notes

      Filtered through a 0.22 µm membrane.

    • Clonality

      Monoclonal

    • Clone number

      RFXAD55A

    • Isotype

      IgG1

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab51376 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
    Notes
    WB
    1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

    Target

    • Function

      Part of the RFX complex that binds to the X-box of MHC II promoters.

    • Tissue specificity

      Ubiquitous.

    • Involvement in disease

      Defects in RFXAP are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.

    • Post-translational
      modifications

      Phosphorylated.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • Regulatory factor X associated protein antibody
        • Regulatory factor X-associated protein antibody
        • RFX associated protein antibody
        • RFX DNA binding complex 36 kDa subunit antibody
        • RFX DNA-binding complex 36 kDa subunit antibody
        • RFX-associated protein antibody
        • RFXAP antibody
        • RFXAP_HUMAN antibody
        see all

      Images

      • Western blot - Anti-RFXAP antibody [RFXAD55A] (ab51376)
        Western blot - Anti-RFXAP antibody [RFXAD55A] (ab51376)
        Anti-RFXAP antibody [RFXAD55A] (ab51376) at 1/100 dilution + HeLa whole cell lysate at 50 µg

        Secondary
        Anti mouse IgG antibody at 1/2500 dilution

        Predicted band size: 28 kDa
        Observed band size: 30 kDa why is the actual band size different from the predicted?

      References (0)

      Publishing research using ab51376? Please let us know so that we can cite the reference in this datasheet.

      ab51376 has not yet been referenced specifically in any publications.

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