Anti-RFXAP antibody [RFXAD55A] (ab51376)
Key features and details
- Mouse monoclonal [RFXAD55A] to RFXAP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-RFXAP antibody [RFXAD55A]
See all RFXAP primary antibodies -
Description
Mouse monoclonal [RFXAD55A] to RFXAP -
Host species
Mouse -
Tested Applications & Species
Application Species WB Human -
Immunogen
Recombinant fragment, corresponding to internal sequence amino acids 50-200 of Human RFXAP
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Positive control
- HeLa whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Filtered through a 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
RFXAD55A -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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Application | Abreviews | Notes |
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WB |
1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
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Notes |
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WB
1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). |
Target
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Function
Part of the RFX complex that binds to the X-box of MHC II promoters. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in RFXAP are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5994 Human
- Omim: 601861 Human
- SwissProt: O00287 Human
- Unigene: 24422 Human
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Alternative names
- Regulatory factor X associated protein antibody
- Regulatory factor X-associated protein antibody
- RFX associated protein antibody
see all
Images
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Anti-RFXAP antibody [RFXAD55A] (ab51376) at 1/100 dilution + HeLa whole cell lysate at 50 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab51376 has not yet been referenced specifically in any publications.