Anti-RFXAP antibody [RFXAD55A] (ab51376)

Product name

Anti-RFXAP antibody [RFXAD55A]
See all RFXAP primary antibodies
Description

Mouse monoclonal [RFXAD55A] to RFXAP
Host species

Mouse
Tested applications

Suitable for: Dot blot, ELISA, WBmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment, corresponding to internal sequence amino acids 50-200 of Human RFXAP
Positive control
- HeLa whole cell lysate

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Purity

Protein G purified
Purification notes

Filtered through a 0.22 µm membrane.
Clonality

Monoclonal
Clone number

RFXAD55A
Isotype

IgG1
Research areas

Our Abpromise guarantee covers the use of ab51376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Dot blot		Use at an assay dependent dilution.
ELISA		Use at an assay dependent dilution.
WB		1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

Function

Part of the RFX complex that binds to the X-box of MHC II promoters.
Tissue specificity

Ubiquitous.
Involvement in disease

Defects in RFXAP are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
Post-translational
modifications

Phosphorylated.
Cellular localization

Nucleus.
Target information above from: UniProt accession O00287 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5994 Human
- Omim: 601861 Human
- SwissProt: O00287 Human
- Unigene: 24422 Human
Alternative names
- Regulatory factor X associated protein antibody
- Regulatory factor X-associated protein antibody
- RFX associated protein antibody
- RFX DNA binding complex 36 kDa subunit antibody
- RFX DNA-binding complex 36 kDa subunit antibody
- RFX-associated protein antibody
- RFXAP antibody
- RFXAP_HUMAN antibody
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ab51376 has not yet been referenced specifically in any publications.

Publishing research using ab51376? Please let us know so that we can cite the reference in this datasheet.

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