Name: Anti-RFXAP antibody [RFXAD55A]
SKU: ab51376

Product name

Anti-RFXAP antibody [RFXAD55A]
See all RFXAP primary antibodies
Description

Mouse monoclonal [RFXAD55A] to RFXAP
Host species

Mouse
Tested applications

Suitable for: Dot blot, ELISA, WBmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment, corresponding to internal sequence amino acids 50-200 of Human RFXAP
Positive control
- HeLa whole cell lysate

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Purity

Protein G purified
Purification notes

Filtered through a 0.22 µm membrane.
Clonality

Monoclonal
Clone number

RFXAD55A
Isotype

IgG1
Research areas

Our Abpromise guarantee covers the use of ab51376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Dot blot		Use at an assay dependent dilution.
ELISA		Use at an assay dependent dilution.
WB		1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

Function

Part of the RFX complex that binds to the X-box of MHC II promoters.
Tissue specificity

Ubiquitous.
Involvement in disease

Defects in RFXAP are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
Post-translational
modifications

Phosphorylated.
Cellular localization

Nucleus.
Target information above from: UniProt accession O00287 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5994 Human
- Omim: 601861 Human
- SwissProt: O00287 Human
- Unigene: 24422 Human
Alternative names
- Regulatory factor X associated protein antibody
- Regulatory factor X-associated protein antibody
- RFX associated protein antibody
- RFX DNA binding complex 36 kDa subunit antibody
- RFX DNA-binding complex 36 kDa subunit antibody
- RFX-associated protein antibody
- RFXAP antibody
- RFXAP_HUMAN antibody
see all

ab51376 has not yet been referenced specifically in any publications.

Publishing research using ab51376? Please let us know so that we can cite the reference in this datasheet.

Anti-RFXAP antibody [RFXAD55A] (ab51376)

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