- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-RFXAP antibody [RFXAD55A]
See all RFXAP primary antibodies -
DescriptionMouse monoclonal [RFXAD55A] to RFXAP
-
Host speciesMouse
-
Tested applicationsSuitable for: Dot blot, ELISA, WBmore details
-
Species reactivityReacts with: Human
-
Immunogen
Recombinant fragment, corresponding to internal sequence amino acids 50-200 of Human RFXAP
-
Positive control
- HeLa whole cell lysate
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
-
Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityProtein G purified
-
Purification notesFiltered through a 0.22 µm membrane.
-
ClonalityMonoclonal
-
Clone numberRFXAD55A
-
IsotypeIgG1
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab51376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | Use at an assay dependent dilution. | |
| ELISA | Use at an assay dependent dilution. | |
| WB | 1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). |
Target
-
FunctionPart of the RFX complex that binds to the X-box of MHC II promoters.
-
Tissue specificityUbiquitous.
-
Involvement in diseaseDefects in RFXAP are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
-
Post-translational
modificationsPhosphorylated. -
Cellular localizationNucleus.
- Information by UniProt
-
Database links
- Entrez Gene: 5994 Human
- Omim: 601861 Human
- SwissProt: O00287 Human
- Unigene: 24422 Human
-
Alternative names
- Regulatory factor X associated protein antibody
- Regulatory factor X-associated protein antibody
- RFX associated protein antibody
see all
Images
-
![Western blot - Anti-RFXAP antibody [RFXAD55A] (ab51376)](https://www.abcam.com/ps/products/51/ab51376/Images/ab51376wb.jpg)
Western blot - Anti-RFXAP antibody [RFXAD55A] (ab51376)Anti-RFXAP antibody [RFXAD55A] (ab51376) at 1/100 dilution + HeLa whole cell lysate at 50 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa (why is the actual band size different from the predicted?)
Protocols
References
ab51376 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"