Anti-RFXAP antibody [RFXAD55A] (ab51376)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RFXAP antibody [RFXAD55A]
See all RFXAP primary antibodies -
Description
Mouse monoclonal [RFXAD55A] to RFXAP -
Host species
Mouse -
Tested applications
Suitable for: Dot blot, ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to internal sequence amino acids 50-200 of Human RFXAP
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Positive control
- HeLa whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Filtered through a 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
RFXAD55A -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab51376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | Use at an assay dependent dilution. | |
| ELISA | Use at an assay dependent dilution. | |
| WB | 1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). |
Target
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Function
Part of the RFX complex that binds to the X-box of MHC II promoters. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in RFXAP are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5994 Human
- Omim: 601861 Human
- SwissProt: O00287 Human
- Unigene: 24422 Human
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Alternative names
- Regulatory factor X associated protein antibody
- Regulatory factor X-associated protein antibody
- RFX associated protein antibody
see all
Images
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![Western blot - Anti-RFXAP antibody [RFXAD55A] (ab51376)](https://www.abcam.com/ps/products/51/ab51376/Images/ab51376wb.jpg)
Western blot - Anti-RFXAP antibody [RFXAD55A] (ab51376)Anti-RFXAP antibody [RFXAD55A] (ab51376) at 1/100 dilution + HeLa whole cell lysate at 50 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
ab51376 has not yet been referenced specifically in any publications.
