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Rabbit polyclonal to RGNEF
Predicted to work with:
Non human primates, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region within amino acids 1655-1705 of Human RGNEF (NP_001171164.1).
HeLa, 293T and Jurkat whole cell lysates.
Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 192 kDa.
Use at 2-10 µg/mg of lysate.
Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger.
Phosphorylated on tyrosine upon stimulation of cells by laminin.
Cytoplasm. Cell membrane. Colocalizes with the microtubule radial and cortical systems.
Information by UniProt
190 kDa guanine nucleotide exchange factor antibody
guanine nucleotide exchange factor, 190-KD antibody
Western blot - Anti-RGNEF antibody (ab157095)
All lanes : Anti-RGNEF antibody (ab157095) at 0.4 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 192 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-RGNEF antibody (ab157095)
Immunoprecipitation. ab157095 at 1 µg/ml staining RGNEF in HeLa whole cell lysate immunoprecipitated using ab157095 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG.
Predicted band size : 192 kDa.
Exposure time: 3 minutes
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"