Key features and details
- Rabbit polyclonal to RGNEF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RGNEF antibody
DescriptionRabbit polyclonal to RGNEF
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Non human primates, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region within amino acids 1655-1705 of Human RGNEF (NP_001171164.1).
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab157095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 192 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionFunctions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
modificationsPhosphorylated on tyrosine upon stimulation of cells by laminin.
Cellular localizationCytoplasm. Cell membrane. Colocalizes with the microtubule radial and cortical systems.
- Information by UniProt
- 190 kDa guanine nucleotide exchange factor antibody
- guanine nucleotide exchange factor, 190-KD antibody
- KIAA1998 antibody
All lanes : Anti-RGNEF antibody (ab157095) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 192 kDa
Exposure time: 3 minutes
Immunoprecipitation. ab157095 at 1 µg/ml staining RGNEF in HeLa whole cell lysate immunoprecipitated using ab157095 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: Control IgG. Predicted band size : 192 kDa. Exposure time: 3 minutes
ab157095 has been referenced in 2 publications.
- Juettner VV et al. VE-PTP stabilizes VE-cadherin junctions and the endothelial barrier via a phosphatase-independent mechanism. J Cell Biol 218:1725-1742 (2019). PubMed: 30948425
- Hawley ZCE et al. Novel miR-b2122 regulates several ALS-related RNA-binding proteins. Mol Brain 10:46 (2017). WB ; Human . PubMed: 28969660