Anti-RGS10 antibody [EPR10657(B)] (ab154172)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-RGS10 antibody [EPR10657(B)]
See all RGS10 primary antibodies -
DescriptionRabbit monoclonal [EPR10657(B)] to RGS10
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide, corresponding to residues of Human RGS10 (UniProt: O43665).
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Positive control
- Jurkat and K562 cell lysates; Human prostate tissue; K562 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR10657(B)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 20 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. |
Target
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FunctionInhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Associates specifically with the activated forms of the G protein subunits G(i)-alpha and G(z)-alpha but fails to interact with the structurally and functionally distinct G(s)-alpha subunit. Activity on G(z)-alpha is inhibited by palmitoylation of the G-protein.
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Sequence similaritiesContains 1 RGS domain.
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Post-translational
modificationsIsoform 3 is phosphorylated on Ser-16. - Information by UniProt
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Database links
- Entrez Gene: 6001 Human
- Omim: 602856 Human
- SwissProt: O43665 Human
- Unigene: 501200 Human
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Alternative names
- Regulator of G protein signaling 10 antibody
- Regulator of G-protein signaling 10 antibody
- RGS10 antibody
- RGS10_HUMAN antibody
Images
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![Western blot - Anti-RGS10 antibody [EPR10657(B)] (ab154172)](https://www.abcam.com/ps/products/154/ab154172/Images/RGS10-Primary-antibodies-ab154172-1.jpg)
Western blot - Anti-RGS10 antibody [EPR10657(B)] (ab154172)All lanes : Anti-RGS10 antibody [EPR10657(B)] (ab154172) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : K562 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 20 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)](https://www.abcam.com/ps/products/154/ab154172/Images/RGS10-Primary-antibodies-ab154172-4.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling RGS10 with ab154172 at 1/50 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] (ab154172)](https://www.abcam.com/ps/products/154/ab154172/Images/RGS10-Primary-antibodies-ab154172-5.gif)
Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] (ab154172)Immunofluorescent analysis of K562 cells labeling RGS10 with ab154172 at 1/50 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)](https://www.abcam.com/ps/products/154/ab154172/Images/RGS10-Primary-antibodies-ab154172-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154172 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)](https://www.abcam.com/ps/products/154/ab154172/Images/RGS10-Primary-antibodies-ab154172-7.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154172 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)](https://www.abcam.com/ps/products/154/ab154172/Images/RGS10-Primary-antibodies-ab154172-8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab154172 showing +ve staining.
Protocols
References
ab154172 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"