Recombinant
RabMAb

Recombinant Anti-RGS10 antibody [EPR10657(B)] (ab154172)

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Overview

  • Product name

    Anti-RGS10 antibody [EPR10657(B)]
    See all RGS10 primary antibodies

  • Description

    Rabbit monoclonal [EPR10657(B)] to RGS10

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide, corresponding to residues of Human RGS10 (UniProt: O43665).

  • Positive control

    • Jurkat and K562 cell lysates; Human prostate tissue; K562 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab154172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Associates specifically with the activated forms of the G protein subunits G(i)-alpha and G(z)-alpha but fails to interact with the structurally and functionally distinct G(s)-alpha subunit. Activity on G(z)-alpha is inhibited by palmitoylation of the G-protein.

    • Sequence similarities

      Contains 1 RGS domain.

    • Post-translational
      modifications

      Isoform 3 is phosphorylated on Ser-16.
    • Information by UniProt

    • Database links

      • Alternative names

        • Regulator of G protein signaling 10 antibody
        • Regulator of G-protein signaling 10 antibody
        • RGS10 antibody
        • RGS10_HUMAN antibody

      Images

      • Western blot - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Western blot - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        All lanes : Anti-RGS10 antibody [EPR10657(B)] (ab154172) at 1/1000 dilution

        Lane 1 : Jurkat cell lysates
        Lane 2 : K562 cell lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 20 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

        Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling RGS10 with ab154172 at 1/50 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Immunofluorescent analysis of K562 cells labeling RGS10 with ab154172 at 1/50 dilution.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

        Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154172 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

        Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154172 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

        Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab154172 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      References

      ab154172 has not yet been referenced specifically in any publications.

      Publishing research using ab154172? Please let us know so that we can cite the reference in this datasheet.

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