RecombinantRabMAb

Recombinant Anti-RGS10 antibody [EPR10657(B)] (ab154172)

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Western blot - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Anti-RGS10 antibody [EPR10657(B)] (ab154172)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10657(B)] to RGS10
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-RGS10 antibody [EPR10657(B)]
    See all RGS10 primary antibodies

  • Description

    Rabbit monoclonal [EPR10657(B)] to RGS10

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide, corresponding to residues of Human RGS10 (UniProt: O43665).

  • Positive control

    • Jurkat and K562 cell lysates; Human prostate tissue; K562 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10657(B)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Associates specifically with the activated forms of the G protein subunits G(i)-alpha and G(z)-alpha but fails to interact with the structurally and functionally distinct G(s)-alpha subunit. Activity on G(z)-alpha is inhibited by palmitoylation of the G-protein.

    • Sequence similarities

      Contains 1 RGS domain.

    • Post-translational
      modifications

      Isoform 3 is phosphorylated on Ser-16.
    • Information by UniProt

    • Database links

      • Alternative names

        • Regulator of G protein signaling 10 antibody
        • Regulator of G-protein signaling 10 antibody
        • RGS10 antibody
        • RGS10_HUMAN antibody

      Images

      • Western blot - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Western blot - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        All lanes : Anti-RGS10 antibody [EPR10657(B)] (ab154172) at 1/1000 dilution

        Lane 1 : Jurkat cell lysates
        Lane 2 : K562 cell lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 20 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

        Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling RGS10 with ab154172 at 1/50 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Immunofluorescent analysis of K562 cells labeling RGS10 with ab154172 at 1/50 dilution.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

        Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154172 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

        Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154172 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

        Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab154172 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-RGS10 antibody [EPR10657(B)] (ab154172)
        Anti-RGS10 antibody [EPR10657(B)] (ab154172)

      References (0)

      Publishing research using ab154172? Please let us know so that we can cite the reference in this datasheet.

      ab154172 has not yet been referenced specifically in any publications.

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