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RecombinantRabMAb

Recombinant Anti-RGS10 antibody [EPR10657(B)] (ab154172)

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Western blot - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
  • Anti-RGS10 antibody [EPR10657(B)] (ab154172)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10657(B)] to RGS10
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-RGS10 antibody [EPR10657(B)]
    See all RGS10 primary antibodies

  • Description

    Rabbit monoclonal [EPR10657(B)] to RGS10

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat and K562 cell lysates; Human prostate tissue; K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR10657(B)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Associates specifically with the activated forms of the G protein subunits G(i)-alpha and G(z)-alpha but fails to interact with the structurally and functionally distinct G(s)-alpha subunit. Activity on G(z)-alpha is inhibited by palmitoylation of the G-protein.

  • Sequence similarities

    Contains 1 RGS domain.

  • Post-translational
    modifications

    Isoform 3 is phosphorylated on Ser-16.
  • Information by UniProt

  • Database links

    • Alternative names

      • Regulator of G protein signaling 10 antibody
      • Regulator of G-protein signaling 10 antibody
      • RGS10 antibody
      • RGS10_HUMAN antibody

    Images

    • Western blot - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Western blot - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      All lanes : Anti-RGS10 antibody [EPR10657(B)] (ab154172) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : K562 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 20 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

      Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling RGS10 with ab154172 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Immunofluorescent analysis of K562 cells labeling RGS10 with ab154172 at 1/50 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

      Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154172 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

      Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154172 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] (ab154172)

      Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab154172 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-RGS10 antibody [EPR10657(B)] (ab154172)
      Anti-RGS10 antibody [EPR10657(B)] (ab154172)

    References (0)

    Publishing research using ab154172? Please let us know so that we can cite the reference in this datasheet.

    ab154172 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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