Key features and details
- Rabbit polyclonal to RGS11
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RGS11 antibody
DescriptionRabbit polyclonal to RGS11
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to a region within internal sequence amino acids 151-248 of Human RGS11 (UniProt ID: O94810).
- 293T, H1299, HeLaS3, MOLT4 and Raji whole cell lysates; HeLa cells; Human NCIN87 xenograft tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 53 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionInhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form.
Sequence similaritiesContains 1 DEP domain.
Contains 1 G protein gamma domain.
Contains 1 RGS domain.
- Information by UniProt
- Regulator of G protein signaling 11 antibody
- Regulator of G protein signalling 11 antibody
- Regulator of G-protein signaling 11 antibody
Anti-RGS11 antibody (ab155676) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 53 kDa
10% SDS PAGE
Immunofluorescence analysis of methanol-fixed HeLa cells labeling RGS11 with ab155676 at 1/500 dilution (green). Lower image shows costaining with Hoechst 33342 (blue).
Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue labeling RGS11 with ab155676 at 1/500 dilution.
ab155676 has not yet been referenced specifically in any publications.