Product nameAnti-RGS16 antibody
See all RGS16 primary antibodies
DescriptionRabbit polyclonal to RGS16
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
- Jurkat cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab49259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 23 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:312500.
FunctionInhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. May play a role in regulating the kinetics of signaling in the phototransduction cascade.
Tissue specificityAbundantly expressed in retina with lower levels of expression in most other tissues.
Sequence similaritiesContains 1 RGS domain.
modificationsPalmitoylated on Cys-2 and/or Cys-12.
Phosphorylation on Tyr-168 upon EGFR stimulation. Enhanced GTPase accelerating (GAP) activity on G(i)-alpha.
- Information by UniProt
- A28 RGS14 antibody
- A28 RGS14P antibody
- A28-RGS14P antibody
Anti-RGS16 antibody (ab49259) at 1 µg/ml + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 23 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
ab49259 has not yet been referenced specifically in any publications.