Key features and details
- Rabbit polyclonal to RGS17 / RGSZ2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RGS17 / RGSZ2 antibody
See all RGS17 / RGSZ2 primary antibodies
DescriptionRabbit polyclonal to RGS17 / RGSZ2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human testis tissue. WB: Transfected HEK293T whole cell lysates.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab150814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 24 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins.
Tissue specificityPredominantly expressed in the cerebellum. Also expressed in the cortex and medulla. Weakly expressed in a number of peripheral tissues notably spleen, lung and leukocytes.
Sequence similaritiesContains 1 RGS domain.
modificationsFatty acylated. Heavily palmitoylated in the cysteine string motif.
N- and O-glycosylated in synapsomal membranes.
Serine phosphorylated in synapsomal membranes.
Sumoylated by SUMO1 and SUM02 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits.
Cellular localizationMembrane. Nucleus. Cytoplasm. Shuttles between the cytoplasm/cell membrane and the nucleus. Anchored to the membrane through palmitoylation.
- Information by UniProt
- hRGS17 antibody
- Regulator of G protein signalling 17 antibody
- Regulator of G protein signalling Z2 antibody
Immunohistochemical analysis of human testis tissue labeling RGS17 / RGSZ2 in the nucleus of a subset of cells in seminiferus ducts with ab150814 at at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-RGS17 / RGSZ2 antibody (ab150814) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : RGS17 over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 24 kDa
ab150814 has not yet been referenced specifically in any publications.