Key features and details
- Rabbit polyclonal to RGS2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RGS2 antibody
See all RGS2 primary antibodies
DescriptionRabbit polyclonal to RGS2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Rhesus monkey
Recombinant fragment within Human RGS2 (internal sequence). The exact sequence is proprietary.
Database link: P41220
- IP: U-87 MG whole cell extract. WB: U-87 MG whole cell extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 24 kDa.|
|IP||1/100 - 1/500.|
FunctionRegulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:11063746, PubMed:19478087). Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle (By similarity). Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein (PubMed:19736320).
Tissue specificityExpressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).
Sequence similaritiesContains 1 RGS domain.
modificationsPhosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity.
Cellular localizationCell membrane. Mitochondrion and Cell membrane. Cytoplasm. Nucleus, nucleolus.
- Information by UniProt
- Basic helix-loop-helix phosphoprotein G0S8 antibody
- Cell growth inhibiting protein 31 antibody
- Cell growth-inhibiting gene 31 protein antibody
RGS2 was immunoprecipitated from U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract with 5 µg ab227251. Western blot was performed from the immunoprecipitate using ab227251. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in U-87 MG whole cell extract.
Lane 2: ab227251 IP in U-87 MG whole cell extract.
Anti-RGS2 antibody (ab227251) at 1/1000 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg
Predicted band size: 24 kDa
12% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227251 has not yet been referenced specifically in any publications.