Product nameAnti-RGS2 antibody
See all RGS2 primary antibodies
DescriptionRabbit polyclonal to RGS2
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Dog, Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human RGS2 aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human RGS2 protein (ab125783) can be used as a positive control in WB. This antibody gave a positive signal in Human Brain Tissue Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab36561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a dilution of 1/250 for 1 hr.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 24 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRegulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:11063746, PubMed:19478087). Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle (By similarity). Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein (PubMed:19736320).
Tissue specificityExpressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).
Sequence similaritiesContains 1 RGS domain.
modificationsPhosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity.
Cellular localizationCell membrane. Mitochondrion and Cell membrane. Cytoplasm. Nucleus, nucleolus.
- Information by UniProt
- Basic helix-loop-helix phosphoprotein G0S8 antibody
- Cell growth inhibiting protein 31 antibody
- Cell growth-inhibiting gene 31 protein antibody
Anti-RGS2 antibody (ab36561) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Additional bands at: 51 kDa (possible cross reactivity)
ICC/IF image of ab36561 stained human SH-SY5Y cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab36561, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab36561 staining RGS2 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
This product has been referenced in:
- Wang C et al. Downregulation of regulator of G protein signaling 2 expression in breast invasive carcinoma of no special type: Clinicopathological associations and prognostic relevance. Oncol Lett 15:213-220 (2018). Read more (PubMed: 29391880) »
- Linder A et al. Analysis of regulator of G-protein signalling 2 (RGS2) expression and function during prostate cancer progression. Sci Rep 8:17259 (2018). Read more (PubMed: 30467386) »