Anti-RGS2 antibody (ab36561)

Reviews (3)Q&A (4)References (8)
Western blot - Anti-RGS2 antibody (ab36561)
  • Immunocytochemistry/ Immunofluorescence - Anti-RGS2 antibody (ab36561)
  • Western blot - Anti-RGS2 antibody (ab36561)

Key features and details

  • Rabbit polyclonal to RGS2
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RGS2 antibody
    See all RGS2 primary antibodies

  • Description

    Rabbit polyclonal to RGS2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog

  • Immunogen

    Synthetic peptide corresponding to Human RGS2 aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab36560)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab36561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
ICC/IF
Notes
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at a dilution of 1/250 for 1 hr.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 24 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

  • Function

    Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:11063746, PubMed:19478087). Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle (By similarity). Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein (PubMed:19736320).

  • Tissue specificity

    Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).

  • Sequence similarities

    Contains 1 RGS domain.

  • Post-translational
    modifications

    Phosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity.

  • Cellular localization

    Cell membrane. Mitochondrion and Cell membrane. Cytoplasm. Nucleus, nucleolus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Basic helix-loop-helix phosphoprotein G0S8 antibody
    • Cell growth inhibiting protein 31 antibody
    • Cell growth-inhibiting gene 31 protein antibody
    • G0 to G1 switch regulatory 8 24kD antibody
    • G0/G1 switch regulatory protein 8 antibody
    • G0S8 antibody
    • GOS8 antibody
    • OTTHUMP00000060765 antibody
    • Regulator of G protein signaling 2 antibody
    • Regulator of G protein signalling 2 24kD antibody
    • Regulator of G-protein signaling 2 antibody
    • Regulators of G protein signaling XRGSVI antibody
    • RGS 2 antibody
    • RGS2 antibody
    • RGS2_HUMAN antibody
    see all

Images

  • Western blot - Anti-RGS2 antibody (ab36561)
    Western blot - Anti-RGS2 antibody (ab36561)
    Anti-RGS2 antibody (ab36561) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?
    Additional bands at: 51 kDa (possible cross reactivity)

  • Immunocytochemistry/ Immunofluorescence - Anti-RGS2 antibody (ab36561)
    Immunocytochemistry/ Immunofluorescence - Anti-RGS2 antibody (ab36561)
    ICC/IF image of ab36561 stained human SH-SY5Y cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab36561, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Western blot - Anti-RGS2 antibody (ab36561)
    Western blot - Anti-RGS2 antibody (ab36561)

References (8)

Publishing research using ab36561? Please let us know so that we can cite the reference in this datasheet.

ab36561 has been referenced in 8 publications.

  • Damasceno S  et al. Top Common Differentially Expressed Genes in the Epileptogenic Nucleus of Two Strains of Rodents Susceptible to Audiogenic Seizures: WAR and GASH/Sal. Front Neurol 11:33 (2020). PubMed: 32117006
  • Wang C  et al. Downregulation of regulator of G protein signaling 2 expression in breast invasive carcinoma of no special type: Clinicopathological associations and prognostic relevance. Oncol Lett 15:213-220 (2018). PubMed: 29391880
  • Linder A  et al. Analysis of regulator of G-protein signalling 2 (RGS2) expression and function during prostate cancer progression. Sci Rep 8:17259 (2018). PubMed: 30467386
  • Bouvet GF  et al. DNA Methylation Regulates RGS2-induced S100A12 Expression in Airway Epithelial Cells. Am J Respir Cell Mol Biol 59:601-613 (2018). PubMed: 29944393
  • Mather Q  et al. A novel protein expression signature differentiates benign lipomas from well-differentiated liposarcomas. Mol Clin Oncol 7:315-321 (2017). PubMed: 28781809
  • de Souza EE  et al. Human Nek7-interactor RGS2 is required for mitotic spindle organization. Cell Cycle 14:656-67 (2015). PubMed: 25664600
  • de Souza EE  et al. Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6. J Proteome Res 13:4074-90 (2014). PubMed: 25093993
  • Subramaniam S  et al. Distinct transcriptional networks in quiescent myoblasts: a role for Wnt signaling in reversible vs. irreversible arrest. PLoS One 8:e65097 (2013). WB ; Mouse . PubMed: 23755177

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-RGS2 antibody

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - nuclear (Brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Lisette Torres

Verified customer

Submitted Aug 30 2018

Western blot abreview for Anti-RGS2 antibody

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (15)
Sample
Dog Cell lysate - whole cell (MDCK)
Specification
MDCK
Blocking step
Milk as blocking agent for 1 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 25°C
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Dr. Edmarcia Elisa Souza

Verified customer

Submitted Mar 07 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RGS2 antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Read More

Mr. Antibody Solutions

Verified customer

Submitted Jan 22 2009

Question

Hi, I would like to test the following product in the indicated application and/or species noted below:

Ab36561 in mouse tissue, with both IHC and in situ ICC.

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Answer

I am very pleased to hear you would like to accept our offer and test ab36561 in mouse. This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse/IHC and ICC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Question

Inquiry: We would like to try your product, anti-RGS2 antibody (ab36561), in mouse tissue. The product description indicates that it is predicted to bind to the mouse protein, however we have not seen evidence that this antibody will work with mouse tissue, in either IHC or ICC. Could you possibly obtain a small sample to test. We would be happy to provide an image for your database/website if we find it to work. Regards.

Read More
Answer

Thank you for contacting us.
Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.
However, since the anti-RGS2 antibody (ab36561) that you referenced has not been tested in mouse tissue, it qualifies for our AbTrial program. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Or you can find more information about this program at the following link:
www.abcam.com/abtrial
We like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.
To find out more about our Abreview system, please see the following link:
https://www.abcam.com/abreviews
Abcam also provides a number of IHC reagents and kits such as DAB substrate kits, donkey or goat sera and antigen retrieval buffers. All of our products are covered by our six month Abpromise guarantee and are fully supported by our scientific support staff. I have provided links to a few of these products below.
DAB substrate kit: https://www.abcam.com/DAB-substrate-kit-ab94665.html
Donkey serum: https://www.abcam.com/Donkey-Serum-ab7475.html
Goat serum: https://www.abcam.com/Goat-Serum-ab7481.html
Antigen retrieval buffer: https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Tris-EDTA-Buffer-pH-9-0-ab93684.html
I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Question

Bonjour,
Nous venons de recevoir des anticorps (ab19932 et ab36561), nous allons les utiliser régulièrement ce mois-ci puis beaucoup moins ensuite.
Est-il possible de les garder à 4°C un peu plus d'un mois sans qu'ils se dégradent pour faciliter l'utilisation et pour éviter de les congeler/décongeler. Il est conseillé sur la notice de les congeler.
Y-a t'il une procédure particulière pour les congeler? On nous a conseillé de mettre du glycérol (de manière à dilué la concentration de l'anticorps par 2) afin qu'il puisse se conserver à -20°C sans congeler.
Êtes-vous d'accord?
Cordialement,

Read More
Answer

Merci de nous avoir contactés.
1) Il est en effet conseillé de stocker les deux anticorps ab19932 et ab36561 à -20 ou -80ºC.
2) Pour éviter les cycles de congélation / décongélation nous recommandons de stocker les anticorps en petits aliquots (minimum 10µl) dans des tubes de type PCR.
Un aliquot décongelé peut ensuite être conservé à +4ºC pendant quelques semaines.
3) Nous déconseillons l'utilisation de glycérol car nous n'avons aucune donnée ni garantie pour ces produits si stockés avec du glycérol.
4) Oui, vous pouvez garder l'anticorps à 4ºC pendant quelques semaines, peut-être également pendant plus d'un mois, car un anticorps purifié est en règle général une molécule robuste et qui reste stable. Nous préférons tout de même une conservation en aliquots congelés.
5) Nous déconseillons dans tous les cas de diluer le produit avant stockage, que ce soit à +4, -20 ou -80ºC.
J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Question

Test ab36561 in Mouse.
Explain the WB images.

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Answer

Merci de nous avoir contactés.
Mes collègues du laboratoire m'ont communiqué l'image du blot obtenu avec les 2 lots de ab36561 actuellement en stock, voir pièce jointe. (Lane 1 : lot GR52442-1, lane 2 : control lane marker, lane 3 : lot# 488871). Ce blot a été obtenu avec des lysats de cerveau humain, 10 µg, référence ab29466 (www.abcam.com/ab29466).
Concernant l'offre de test : puisque le ab36561 n’a pas encore été testé chez la souris, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat d'un anticorps primaire pourra être utilisable lors d’une prochaine commande.
Etapes à suivre :
1. Nous confirmer que vous souhaiteriez tester ab36561 chez la souris afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab36561
2. Acheter ab36561 par téléphone, fax ou internet (www.abcam.com)
3. Le tester chez la souris
4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews
5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.
Même si ab36561 ne pas fonctionne pas chez la souris, après soumission de votre Abreview, vous recevrez quand même une remise sur l’achat de votre prochain anticorps.
N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.
Termes et Conditions : www.abcam.com/collaborationdiscount.
J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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