Overview

  • Product name
  • Description
    Rabbit polyclonal to RGS2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human RGS2.

    Read Abcam's proprietary immunogen policy (Peptide available as ab36560.)

  • Positive control
    • Recombinant Human RGS2 protein (ab125783) can be used as a positive control in WB. This antibody gave a positive signal in Human Brain Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab36561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-P
  • Application notes
    ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: Use at a dilution of 1/250 for 1 hr.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 24 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:11063746, PubMed:19478087). Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle (By similarity). Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein (PubMed:19736320).
    • Tissue specificity
      Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).
    • Sequence similarities
      Contains 1 RGS domain.
    • Post-translational
      modifications
      Phosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity.
    • Cellular localization
      Cell membrane. Mitochondrion and Cell membrane. Cytoplasm. Nucleus, nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Basic helix-loop-helix phosphoprotein G0S8 antibody
      • Cell growth inhibiting protein 31 antibody
      • Cell growth-inhibiting gene 31 protein antibody
      • G0 to G1 switch regulatory 8 24kD antibody
      • G0/G1 switch regulatory protein 8 antibody
      • G0S8 antibody
      • GOS8 antibody
      • OTTHUMP00000060765 antibody
      • Regulator of G protein signaling 2 antibody
      • Regulator of G protein signalling 2 24kD antibody
      • Regulator of G-protein signaling 2 antibody
      • Regulators of G protein signaling XRGSVI antibody
      • RGS 2 antibody
      • RGS2 antibody
      • RGS2_HUMAN antibody
      see all

    Images

    • Anti-RGS2 antibody (ab36561) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 24 kDa
      Observed band size: 23 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 51 kDa (possible cross reactivity)

    • ICC/IF image of ab36561 stained human SH-SY5Y cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab36561, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    • ab36561 staining RGS2 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

      See Abreview

    References

    This product has been referenced in:
    • Mather Q  et al. A novel protein expression signature differentiates benign lipomas from well-differentiated liposarcomas. Mol Clin Oncol 7:315-321 (2017). Read more (PubMed: 28781809) »
    • de Souza EE  et al. Human Nek7-interactor RGS2 is required for mitotic spindle organization. Cell Cycle 14:656-67 (2015). Read more (PubMed: 25664600) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-7 of 7 Abreviews or Q&A

    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - nuclear (Brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    15 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Lisette Torres

    Verified customer

    Submitted Aug 30 2018

    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (15)
    Sample
    Dog Cell lysate - whole cell (MDCK)
    Specification
    MDCK
    Blocking step
    Milk as blocking agent for 1 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Edmarcia Elisa Souza

    Verified customer

    Submitted Mar 07 2014

    Answer

    I am very pleased to hear you would like to accept our offer and test ab36561 in mouse. This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse/IHC and ICC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

    Read More

    Answer

    Thank you for contacting us.
    Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.
    However, since the anti-RGS2 antibody (ab36561) that you referenced has not been tested in mouse tissue, it qualifies for our AbTrial program. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Or you can find more information about this program at the following link:
    www.abcam.com/abtrial
    We like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.
    To find out more about our Abreview system, please see the following link:
    https://www.abcam.com/abreviews
    Abcam also provides a number of IHC reagents and kits such as DAB substrate kits, donkey or goat sera and antigen retrieval buffers. All of our products are covered by our six month Abpromise guarantee and are fully supported by our scientific support staff. I have provided links to a few of these products below.
    DAB substrate kit: https://www.abcam.com/DAB-substrate-kit-ab94665.html
    Donkey serum: https://www.abcam.com/Donkey-Serum-ab7475.html
    Goat serum: https://www.abcam.com/Goat-Serum-ab7481.html
    Antigen retrieval buffer: https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Tris-EDTA-Buffer-pH-9-0-ab93684.html
    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Answer

    Merci de nous avoir contactés.
    1) Il est en effet conseillé de stocker les deux anticorps ab19932 et ab36561 à -20 ou -80ºC.
    2) Pour éviter les cycles de congélation / décongélation nous recommandons de stocker les anticorps en petits aliquots (minimum 10µl) dans des tubes de type PCR.
    Un aliquot décongelé peut ensuite être conservé à +4ºC pendant quelques semaines.
    3) Nous déconseillons l'utilisation de glycérol car nous n'avons aucune donnée ni garantie pour ces produits si stockés avec du glycérol.
    4) Oui, vous pouvez garder l'anticorps à 4ºC pendant quelques semaines, peut-être également pendant plus d'un mois, car un anticorps purifié est en règle général une molécule robuste et qui reste stable. Nous préférons tout de même une conservation en aliquots congelés.
    5) Nous déconseillons dans tous les cas de diluer le produit avant stockage, que ce soit à +4, -20 ou -80ºC.
    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Question
    Answer

    Merci de nous avoir contactés.
    Mes collègues du laboratoire m'ont communiqué l'image du blot obtenu avec les 2 lots de ab36561 actuellement en stock, voir pièce jointe. (Lane 1 : lot GR52442-1, lane 2 : control lane marker, lane 3 : lot# 488871). Ce blot a été obtenu avec des lysats de cerveau humain, 10 µg, référence ab29466 (www.abcam.com/ab29466).
    Concernant l'offre de test : puisque le ab36561 n’a pas encore été testé chez la souris, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat d'un anticorps primaire pourra être utilisable lors d’une prochaine commande.
    Etapes à suivre :
    1. Nous confirmer que vous souhaiteriez tester ab36561 chez la souris afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab36561
    2. Acheter ab36561 par téléphone, fax ou internet (www.abcam.com)
    3. Le tester chez la souris
    4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews
    5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.
    Même si ab36561 ne pas fonctionne pas chez la souris, après soumission de votre Abreview, vous recevrez quand même une remise sur l’achat de votre prochain anticorps.
    N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.
    Termes et Conditions : www.abcam.com/collaborationdiscount.
    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (heart)
    Specification
    heart
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
    Permeabilization
    No
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

    Mr. Antibody Solutions

    Verified customer

    Submitted Jan 22 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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