• Product name
  • Description
    Rabbit polyclonal to RGS2
  • Host species
  • Specificity
    PPlease note that a patterns of "background" bands may be present, this varies from tissue to tissue.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:


    , corresponding to N terminal amino acids 29-44 of Human RGS2.



Our Abpromise guarantee covers the use of ab9963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/15000. Predicted molecular weight: 25 kDa.


  • Function
    Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:11063746, PubMed:19478087). Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle (By similarity). Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein (PubMed:19736320).
  • Tissue specificity
    Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).
  • Sequence similarities
    Contains 1 RGS domain.
  • Post-translational
    Phosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity.
  • Cellular localization
    Cell membrane. Mitochondrion and Cell membrane. Cytoplasm. Nucleus, nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic helix-loop-helix phosphoprotein G0S8 antibody
    • Cell growth inhibiting protein 31 antibody
    • Cell growth-inhibiting gene 31 protein antibody
    • G0 to G1 switch regulatory 8 24kD antibody
    • G0/G1 switch regulatory protein 8 antibody
    • G0S8 antibody
    • GOS8 antibody
    • OTTHUMP00000060765 antibody
    • Regulator of G protein signaling 2 antibody
    • Regulator of G protein signalling 2 24kD antibody
    • Regulator of G-protein signaling 2 antibody
    • Regulators of G protein signaling XRGSVI antibody
    • RGS 2 antibody
    • RGS2 antibody
    • RGS2_HUMAN antibody
    see all


This product has been referenced in:
  • Snabaitis AK  et al. Regulation of the extracellular signal-regulated kinase pathway in adult myocardium: differential roles of G(q/11), Gi and G(12/13) proteins in signalling by alpha1-adrenergic, endothelin-1 and thrombin-sensitive protease-activated receptors. Cell Signal 17:655-64 (2005). WB ; Rat . Read more (PubMed: 15683740) »
  • Kveberg L  et al. Expression of regulator of G protein signalling proteins in natural killer cells, and their modulation by Ly49A and Ly49D. Immunology 115:358-65 (2005). Read more (PubMed: 15946253) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. I am sorry to hear that the antibody is giving you problems. As we discussed over the phone, potential troubleshooting tips that could help improve your results are: 1) loading more protein per lane (up to 100 ug max...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Cell lysate - whole cell (mouse ventricle)
Loading amount
50 µg
mouse ventricle
Gel Running Conditions
Reduced Denaturing (12% gradient gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Serban Georgescu

Verified customer

Submitted Apr 21 2008

I received confirmation today that the patterns of "background" bands varies from tissue to tissue. The VTA area of the brain has not been tested in the tester's hands therefore we do not have experience of a 12kDa band, but in other tissues other band...

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Thank you for taking the time to submit these concerns to me. If there are only two bands on the blot and background this problem may be due in part to target breakdown or standard cross reactivity due to the fact that the product is a pAb. Since pAbs ...

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Thank you for your patience. The originator of the antibody has never looked at 3T3 cells (which are fibroblasts), but at the RNA level RGS2 is most highly expressed in brain. In 3T3 cells, RGS2 mRNA may be low, or the protein may be unstable. The l...

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Thank you for your phone call. I'm sending you a free of charge replacement for ab9963, for your record it is on order# 50879 and you should receive it Tuesday. The vial is from lot 27062 but I checked it and it is fine. Please let me know if you have ...

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Thank you for your enquiry. Ab9963 has been tested only against transfected or purified recombinant RGS2. If you have any more questions, please contact us again.

Thank you for your patience. Unfortunately I still have not heard from the originator of this antibody regarding your questions about the multiple bands. As for the background that you are experiencing, high background is usually a sign for blocking pr...

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Thank you for your enquiry and your interest in our products. All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). Our policy at Abcam is that if an ant...

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Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

1-10 of 14 Abreviews or Q&A


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