Product nameRGS2 overexpression 293T lysate (whole cell)
ab94228 is a 293T cell transfected lysate in which Human RGS2 has been transiently over-expressed using a pCMV-RGS2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:11063746, PubMed:19478087). Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle (By similarity). Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein (PubMed:19736320). PTM: Phosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity. Similarity: Contains 1 RGS domain. Tissue specificity: Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).
Our Abpromise guarantee covers the use of ab94228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94228 at 15μg/lane on an SDS-PAGE gel
All lanes : Anti-RGS2 antibody (ab57922) at 1/500 dilution
Lane 1 :
RGS2 overexpression 293T lysate (whole cell) (ab94228)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94228 has not yet been referenced specifically in any publications.