Overview

  • Product name

  • Description

    Rabbit polyclonal to RGS20
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of RGS20
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RGS20. The exact sequence is proprietary.
    Database link: O76081

  • Positive control

    • HEK293T, NIH3T3, H9C2 whole cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab191500 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen, and the purity is > 95% (by SDS-PAGE).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.

Target

  • Function

    Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins.
  • Tissue specificity

    Isoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.
  • Sequence similarities

    Contains 1 RGS domain.
  • Post-translational
    modifications

    Fatty acylated. Heavily palmitoylated in the cysteine string motif.
    N- and O-glycosylated in synapsomal membranes.
    Serine phosphorylated in synapsomal membranes.
    Sumoylated by SUMO1 and SUM02 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits.
  • Cellular localization

    Membrane. Nucleus. Cytoplasm. Shuttles between the cytoplasm/cell membrane and the nucleus (By similarity). Anchored to the membrane through palmitoylation.
  • Information by UniProt
  • Database links

  • Alternative names

    • G(z)GAP antibody
    • Gz selective GTPase activating protein antibody
    • Gz-GAP antibody
    • Gz-selective GTPase-activating protein antibody
    • HGNC:14600 antibody
    • Regulator of G protein signaling 20 antibody
    • Regulator of G-protein signaling 20 antibody
    • Regulator of G-protein signaling Z1 antibody
    • Regulator of Gz selective protein signaling 1 antibody
    • Regulator of Gz-selective protein signaling 1 antibody
    • RGS20 antibody
    • RGS20_HUMAN antibody
    • RGSZ1 antibody
    • ZGAP1 antibody
    see all

Images

  • All lanes : Anti-RGS20 antibody (ab191500)

    Lane 1 : HEK293T whole cell lysate
    Lane 2 : NIH 3T3 whole cell lysate
    Lane 3 : H9C2 whole cell lysate

    Predicted band size: 43 kDa

References

ab191500 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191500.
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