Key features and details
- Rabbit polyclonal to RGS20
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-RGS20 antibody
See all RGS20 primary antibodies
DescriptionRabbit polyclonal to RGS20
SpecificityThis antibody detects endogenous levels of RGS20
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human RGS20. The exact sequence is proprietary.
Database link: O76081
- HEK293T, NIH3T3, H9C2 whole cell lysate
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab191500 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen, and the purity is > 95% (by SDS-PAGE).
Our Abpromise guarantee covers the use of ab191500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.|
FunctionInhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins.
Tissue specificityIsoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.
Sequence similaritiesContains 1 RGS domain.
modificationsFatty acylated. Heavily palmitoylated in the cysteine string motif.
N- and O-glycosylated in synapsomal membranes.
Serine phosphorylated in synapsomal membranes.
Sumoylated by SUMO1 and SUM02 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits.
Cellular localizationMembrane. Nucleus. Cytoplasm. Shuttles between the cytoplasm/cell membrane and the nucleus (By similarity). Anchored to the membrane through palmitoylation.
- Information by UniProt
- G(z)GAP antibody
- Gz selective GTPase activating protein antibody
- Gz-GAP antibody
ab191500 has been referenced in 2 publications.
- Li G et al. Regulator of G protein signaling 20 promotes proliferation and migration in bladder cancer via NF-?B signaling. Biomed Pharmacother 117:109112 (2019). PubMed: 31212130
- Huang G et al. lncRNA NEAT1 promotes cell proliferation and invasion by regulating miR-365/RGS20 in oral squamous cell carcinoma. Oncol Rep 39:1948-1956 (2018). PubMed: 29484420