Overview

  • Product name

    Anti-RGS3 antibody - C-terminal
    See all RGS3 primary antibodies
  • Description

    Rabbit polyclonal to RGS3 - C-terminal
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse RGS3 aa 909-925 (C terminal).
    Sequence:

    DSYTREHTKENLQSITR


    Database link: Q9DC04

  • Positive control

    • Rat testis, rat brain rat spleen, HeLa and U87 cell lysates; rat intestine tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 106 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 PDZ (DHR) domain.
    Contains 1 RGS domain.
  • Post-translational
    modifications

    Phosphorylated by cyclic GMP-dependent protein kinase.
    ISGylated.
  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. Long isoforms are cytoplasmic and associated with the plasma membrane. Short isoforms are nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • C2PA antibody
    • FLJ20370 antibody
    • FLJ31516 antibody
    • FLJ90496 antibody
    • PDZ RGS 3 antibody
    • PDZ RGS3 antibody
    • Regulator of G protein signaling 3 antibody
    • Regulator of G protein signalling 3 antibody
    • Regulator of G-protein signaling 3 antibody
    • RGP 3 antibody
    • RGP3 antibody
    • RGS 3 antibody
    • RGS3 antibody
    • RGS3_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded rat intestine tissue labeling RGS3 with ab191047 at 1 µg/ml.

  • All lanes : Anti-RGS3 antibody - C-terminal (ab191047) at 0.5 µg/ml

    Lane 1 : Rat testis tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat spleen tissue lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : U87 cell lysate

    Predicted band size: 106 kDa

References

ab191047 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191047.
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