Product nameRGS3 overexpression 293T lysate (whole cell)
ab94242 is a 293T cell transfected lysate in which Human RGS3 has been transiently over-expressed using a pCMV-RGS3 Full length plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailes about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases. Similarity: Contains 1 C2 domain. Contains 1 PDZ (DHR) domain. Contains 1 RGS domain. PTM: Phosphorylated by cyclic GMP-dependent protein kinase. ISGylated.
Our Abpromise guarantee covers the use of ab94242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94242 at 15μg/lane on an SDS-PAGE gel.
All lanes : Anti-RGS3 antibody (ab57923) at 1/500 dilution
Lane 1 :
RGS3 overexpression 293T lysate (whole cell) (ab94242)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94242 has not yet been referenced specifically in any publications.