For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    rgs4-antibody-ab97307.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Regulators
Share by email

Anti-RGS4 antibody (ab97307)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS4 antibody (ab97307)
  • Western blot - Anti-RGS4 antibody (ab97307)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS4 antibody (ab97307)
  • Western blot - Anti-RGS4 antibody (ab97307)
  • Western blot - Anti-RGS4 antibody (ab97307)

Key features and details

  • Rabbit polyclonal to RGS4
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human RGS4 protein (ab113136)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-RGS4 antibody
  • Description

    Rabbit polyclonal to RGS4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-198 of Human RGS4 (AAH00737)

  • Positive control

    • WB: Raji cell lysate, rat and mouse brain tissue extract; IHC-P: Human breast cancer and mouse muscle tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Regulators
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human RGS4 protein (ab113136)

Applications

Our Abpromise guarantee covers the use of ab97307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 23 kDa.

Target

  • Function

    Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein.
  • Involvement in disease

    Genetic variation in RGS4 is associated with susceptibility to schizophrenia type 9 (SCZD9) [MIM:604906]. A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder.
  • Sequence similarities

    Contains 1 RGS domain.
  • Post-translational
    modifications

    Palmitoylated on Cys-2 and/or Cys-12.
    Phosphorylated by cyclic GMP-dependent protein kinase.
  • Target information above from: UniProt accession P49798 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5999 Human
    • Entrez Gene: 19736 Mouse
    • Entrez Gene: 29480 Rat
    • Omim: 602516 Human
    • SwissProt: P49798 Human
    • SwissProt: O08899 Mouse
    • SwissProt: P49799 Rat
    • Unigene: 386726 Human
    • Unigene: 41642 Mouse
    • Unigene: 11065 Rat
    see all
  • Alternative names

    • MGC2124 antibody
    • MGC60244 antibody
    • Regulator of G protein signalling 4 antibody
    • Regulator of G-protein signaling 4 antibody
    • RGP 4 antibody
    • RGP4 antibody
    • RGS 4 antibody
    • RGS4 antibody
    • RGS4_HUMAN antibody
    • Schizophrenia disorder 9 antibody
    • SCZD 9 antibody
    • SCZD9 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS4 antibody (ab97307)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS4 antibody (ab97307)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue staining RGS4 with ab97307 at 1:500 dilution.
    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

  • Western blot - Anti-RGS4 antibody (ab97307)
    Western blot - Anti-RGS4 antibody (ab97307)
    Anti-RGS4 antibody (ab97307) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 23 kDa



    12% SDS-PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS4 antibody (ab97307)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS4 antibody (ab97307)

    Immunohistochemical analysis of paraffin-embedded mouse muscle tissue staining RGS4 with ab97307 at 1:500 dilution.
    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

  • Western blot - Anti-RGS4 antibody (ab97307)
    Western blot - Anti-RGS4 antibody (ab97307)
    Anti-RGS4 antibody (ab97307) at 1/1000 dilution + Mouse cerebellum tissue extract at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 23 kDa



    12% SDS-PAGE

  • Western blot - Anti-RGS4 antibody (ab97307)
    Western blot - Anti-RGS4 antibody (ab97307)
    Anti-RGS4 antibody (ab97307) at 1/1000 dilution + Rat brain tissue extract at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 23 kDa



    12% SDS-PAGE

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab97307? Please let us know so that we can cite the reference in this datasheet.

    ab97307 has been referenced in 1 publication.

    • Xiao D & Gao HX Mechanism of miR-107-targeting of regulator of G-protein signaling 4 in hepatocellular carcinoma. Oncol Lett 18:5145-5154 (2019). PubMed: 31612026

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab97307.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.