Anti-RGS5 antibody (ab196799)
Key features and details
- Rabbit polyclonal to RGS5
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RGS5 antibody
See all RGS5 primary antibodies -
Description
Rabbit polyclonal to RGS5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human RGS5 (internal sequence). The exact sequence is proprietary.
Database link: O15539 -
Positive control
- Human breast carcinoma tissue. Extracts from NIH/3T3.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/50 - 1/100.
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WB |
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
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Notes |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/3000. Predicted molecular weight: 21 kDa. |
Target
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Function
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. -
Sequence similarities
Contains 1 RGS domain. - Information by UniProt
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Database links
- Entrez Gene: 8490 Human
- Entrez Gene: 19737 Mouse
- Entrez Gene: 54294 Rat
- Omim: 603276 Human
- SwissProt: O15539 Human
- SwissProt: O08850 Mouse
- SwissProt: P49800 Rat
- Unigene: 24950 Human
see all -
Alternative names
- MST092 antibody
- MST106 antibody
- MST129 antibody
see all
Images
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human breast carcinoma tissue sections labeling RGS5 with ab196799 at a 1/50 dilution. Right image treated with sythesized peptide.
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All lanes : Anti-RGS5 antibody (ab196799) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3 cells
Lane 2 : Extracts from NIH/3T3 cells with synthesized peptide
Predicted band size: 21 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab196799 has been referenced in 7 publications.
- Li X et al. Implications of regulator of G-protein signaling 5 expression in the pathogenesis of primary and secondary hyperparathyroidism. BMC Endocr Disord 22:156 (2022). PubMed: 35681135
- Liu B et al. Single-cell RNA sequencing reveals the cellular and molecular changes that contribute to the progression of lung adenocarcinoma. Front Cell Dev Biol 10:927300 (2022). PubMed: 36046337
- Wang Y et al. Single-cell analysis of pancreatic ductal adenocarcinoma identifies a novel fibroblast subtype associated with poor prognosis but better immunotherapy response. Cell Discov 7:36 (2021). PubMed: 34035226
- Affo S et al. Promotion of cholangiocarcinoma growth by diverse cancer-associated fibroblast subpopulations. Cancer Cell 39:866-882.e11 (2021). PubMed: 33930309
- Ito Y et al. NKX2-1 re-expression induces cell death through apoptosis and necrosis in dedifferentiated thyroid carcinoma cells. PLoS One 16:e0259558 (2021). PubMed: 34748583
- Wang D et al. RGS5 decreases the proliferation of human ovarian carcinoma-derived primary endothelial cells through the MAPK/ERK signaling pathway in hypoxia. Oncol Rep 41:165-177 (2019). PubMed: 30365142
- Dobie R et al. Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis. Cell Rep 29:1832-1847.e8 (2019). PubMed: 31722201