Key features and details
- Rabbit polyclonal to RGS5
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RGS5 antibody
See all RGS5 primary antibodies
DescriptionRabbit polyclonal to RGS5
SpecificityThis antibody detects endogenous levels of total RGS5 protein.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide within Human RGS5 (internal sequence). The exact sequence is proprietary.
Database link: O15539
- Human breast carcinoma tissue. Extracts from NIH/3T3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/3000. Predicted molecular weight: 21 kDa.|
FunctionInhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha.
Sequence similaritiesContains 1 RGS domain.
- Information by UniProt
- MST092 antibody
- MST106 antibody
- MST129 antibody
All lanes : Anti-RGS5 antibody (ab196799) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3 cells
Lane 2 : Extracts from NIH/3T3 cells with synthesized peptide
Predicted band size: 21 kDa
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human breast carcinoma tissue sections labeling RGS5 with ab196799 at a 1/50 dilution. Right image treated with sythesized peptide.
ab196799 has been referenced in 2 publications.
- Wang D et al. RGS5 decreases the proliferation of human ovarian carcinoma-derived primary endothelial cells through the MAPK/ERK signaling pathway in hypoxia. Oncol Rep 41:165-177 (2019). PubMed: 30365142
- Dobie R et al. Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis. Cell Rep 29:1832-1847.e8 (2019). PubMed: 31722201