Overview

  • Product name

  • Description

    Rabbit polyclonal to RGS6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human RGS6 aa 177-262.
    Database link: P49758

  • Positive control

    • IHC-P: Human colon cancer and melanoma tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab236787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(o)-alpha is specifically enhanced by the RGS6/Gbeta5 dimer.
  • Sequence similarities

    Contains 1 DEP domain.
    Contains 1 G protein gamma domain.
    Contains 1 RGS domain.
  • Cellular localization

    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ43552 antibody
    • G protein signaling 6 regulator antibody
    • GAP antibody
    • GTPase activating protein antibody
    • MGC142132 antibody
    • Regulator of G protein signaling 6 antibody
    • Regulator of G protein signalling 6 antibody
    • Regulator of G-protein signaling 6 antibody
    • RGS 6 antibody
    • RGS6 antibody
    • RGS6_HUMAN antibody
    • S914 antibody
    see all

Images

  • 4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RGS6 (green) using ab236787 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary anitbody. Counterstained with DAPI.

     

  • Paraffin-embedded human colon cancer tissue stained for RGS6 using ab236787 at 1/300 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human melanoma tissue stained for RGS6 using ab236787 at 1/300 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

References

ab236787 has not yet been referenced specifically in any publications.

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