Anti-RGS7 antibody (ab228618)
Key features and details
- Rabbit polyclonal to RGS7
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RGS7 antibody -
Description
Rabbit polyclonal to RGS7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow -
Immunogen
Recombinant fragment within Human RGS7 (internal sequence). The exact sequence is proprietary.
Database link: P49802 -
Positive control
- WB: U-87 MG and SK-N-SH whole cell extracts. ICC/IF: NTERA-2 cl.D1 [NT2/D1] cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa. |
Target
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Relevance
RGS7 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP bound form. Activity on G(o) alpha is specifically enhanced by the RGS6/GNG5 dimer. RGS7 may play a role in synaptic vesicle exocytosis and may play an important role in the rapid regulation of neuronal excitability and the cellular responses to short lived stimulations. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 395620 Chicken
- Entrez Gene: 281452 Cow
- Entrez Gene: 6000 Human
- Entrez Gene: 24012 Mouse
- Entrez Gene: 54296 Rat
- Omim: 602517 Human
- SwissProt: O46470 Cow
- SwissProt: P49802 Human
see all -
Alternative names
- OTTHUMP00000037994 antibody
- Regulator of G protein signalling 7 antibody
- Regulator of G-protein signaling 7 antibody
see all
Images
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All lanes : Anti-RGS7 antibody (ab228618) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa10% SDS-PAGE gel.
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Ice-cold methanol-fixed NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells stained for RGS7 (green) using ab228618 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228618 has not yet been referenced specifically in any publications.