Product nameAnti-RGS7 antibody
See all RGS7 primary antibodies
DescriptionRabbit polyclonal to RGS7
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
Recombinant fragment within Human RGS7 (internal sequence). The exact sequence is proprietary.
Database link: P49802
- WB: U-87 MG and SK-N-SH whole cell extracts. ICC/IF: NTERA-2 cl.D1 [NT2/D1] cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 58 kDa.|
RelevanceRGS7 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP bound form. Activity on G(o) alpha is specifically enhanced by the RGS6/GNG5 dimer. RGS7 may play a role in synaptic vesicle exocytosis and may play an important role in the rapid regulation of neuronal excitability and the cellular responses to short lived stimulations.
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All lanes : Anti-RGS7 antibody (ab228618) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
10% SDS-PAGE gel.
Ice-cold methanol-fixed NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells stained for RGS7 (green) using ab228618 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
ab228618 has not yet been referenced specifically in any publications.