Overview

  • Product name

  • Description

    Rabbit polyclonal to RGS7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment within Human RGS7 (internal sequence). The exact sequence is proprietary.
    Database link: P49802

  • Positive control

    • WB: U-87 MG and SK-N-SH whole cell extracts. ICC/IF: NTERA-2 cl.D1 [NT2/D1] cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.

Target

  • Relevance

    RGS7 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP bound form. Activity on G(o) alpha is specifically enhanced by the RGS6/GNG5 dimer. RGS7 may play a role in synaptic vesicle exocytosis and may play an important role in the rapid regulation of neuronal excitability and the cellular responses to short lived stimulations.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • OTTHUMP00000037994 antibody
    • Regulator of G protein signalling 7 antibody
    • Regulator of G-protein signaling 7 antibody
    • Regulator of G-protein signaling RGS7 antibody
    • Regulator of G-protein signalling 7 antibody
    see all

Images

  • All lanes : Anti-RGS7 antibody (ab228618) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 58 kDa



    10% SDS-PAGE gel.

  • Ice-cold methanol-fixed NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells stained for RGS7 (green) using ab228618 at 1/500 dilution in ICC/IF.

    Nuclear counter stain: Hoechst 33342 (blue).

References

ab228618 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228618.
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