1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Regulator of G protein-coupled receptor (GPCR) signaling. Regulatory subunit of the R7-Gbeta5 complexes that acts by controlling the subcellular location of the R7-Gbeta5 complexes. When palmitoylated, it targets the R7-Gbeta5 complexes to the plasma membrane, leading to inhibit G protein alpha subunits. When it is unpalmitoylated, the R7-Gbeta5 complexes undergo a nuclear/cypolasmic shuttling. May also act by controlling the proteolytic stability of R7 proteins, probably by protecting them from degradation
Nucleus. Cytoplasm. Cell membrane; Lipid-anchor. Note: Shuttling between the plasma membrane, the cytoplasm and the nucleus is regulated by palmitoylation