Overview

  • Product name

    Anti-RGS9 antibody [EPR2873]
    See all RGS9 primary antibodies
  • Description

    Rabbit monoclonal [EPR2873] to RGS9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within RGS9. The exact sequence is proprietary.

  • Positive control

    • WB: Y79 and PC12 cell lysates and human and mouse spleen tissue lysates. ICC/IF: Neuro-2a cells. Flow Cyt: PC12 and Y79 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2873
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 77 kDa).
Flow Cyt 1/30 - 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction.
    • Tissue specificity

      Highly expressed in the caudate and putamen, lower levels found in the hypothalamus and nucleus accumbens and very low levels in cerebellum. Not expressed in globus pallidus or cingulate cortex. Isoform 2 is expressed predominantly in pineal gland and retina. Isoform 3 is expressed in retina (abundant in photoreceptors).
    • Involvement in disease

      Defects in RGS9 are a cause of prolonged electroretinal response suppression (PERRS) [MIM:608415]; also known as bradyopsia. PERRS is characterized by difficulty adjusting to sudden changes in luminance levels mediated by cones.
    • Sequence similarities

      Contains 1 DEP domain.
      Contains 1 G protein gamma domain.
      Contains 1 RGS domain.
    • Domain

      In photoreceptor cells the DEP domain is essential for targeting RGS9 to the outer rod segments.
    • Post-translational
      modifications

      Retinal isoform 3 is light-dependent phosphorylated at 'Ser-478'. Phosphorylation is decreased by light exposition.
    • Cellular localization

      Membrane. Isoform 3 is targeted to the membrane via its interaction with RGS9BP.
    • Information by UniProt
    • Database links

    • Alternative names

      • PERRS antibody
      • R9AP antibody
      • Regulator of G protein signalling 9 antibody
      • Regulator of G-protein signaling 9 antibody
      • RGS9 antibody
      • RGS9_HUMAN antibody
      • RGS9L antibody
      see all

    Images

    • All lanes : Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (purified)

      Lane 1 : Y79 whole cell lysate
      Lane 2 : Mouse spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 77 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.
    • Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (purified) + PC-12 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 77 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.
    • Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling RGS9 with purified ab108975 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • Flow Cytometry analysis of Y79 cells labelling RGS9 with purified ab108975 at a dilution of 1/60 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • All lanes : Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (unpurified)

      Lane 1 : PC12 lysates
      Lane 2 : Human spleen lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 77 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?



      Note: Predicted molecular weights for UniProt ID O75916 isoforms 1 to 4 are ~77, 55, 57 and 50 kDa respectively. Therefore, the observed WB band may correspond to isoform 2, 3 or 4.
    • Flow cytometric analysis of permeabilized PC12 cells labelling RGS9 with unpurified ab108975 (red) at a dilution of 1/30 or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Vogt A  et al. Isotope coded protein labeling coupled immunoprecipitation (ICPL-IP): a novel approach for quantitative protein complex analysis from native tissue. Mol Cell Proteomics 12:1395-406 (2013). Read more (PubMed: 23268931) »
    See 1 Publication for this product

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