RecombinantRabMAb

Recombinant Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)

Submit a review Submit a question
Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
  • Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
  • Immunocytochemistry - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
  • Flow Cytometry - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
  • Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
  • Flow Cytometry - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
  • Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2873] to RGS9 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RGS9 antibody [EPR2873] - BSA and Azide free
    See all RGS9 primary antibodies

  • Description

    Rabbit monoclonal [EPR2873] to RGS9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within RGS9. The exact sequence is proprietary.

  • General notes

    Ab247728 is the carrier-free version of ab108975. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247728 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab247728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 77 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction.

    • Tissue specificity

      Highly expressed in the caudate and putamen, lower levels found in the hypothalamus and nucleus accumbens and very low levels in cerebellum. Not expressed in globus pallidus or cingulate cortex. Isoform 2 is expressed predominantly in pineal gland and retina. Isoform 3 is expressed in retina (abundant in photoreceptors).

    • Involvement in disease

      Defects in RGS9 are a cause of prolonged electroretinal response suppression (PERRS) [MIM:608415]; also known as bradyopsia. PERRS is characterized by difficulty adjusting to sudden changes in luminance levels mediated by cones.

    • Sequence similarities

      Contains 1 DEP domain.
      Contains 1 G protein gamma domain.
      Contains 1 RGS domain.

    • Domain

      In photoreceptor cells the DEP domain is essential for targeting RGS9 to the outer rod segments.

    • Post-translational
      modifications

      Retinal isoform 3 is light-dependent phosphorylated at 'Ser-478'. Phosphorylation is decreased by light exposition.

    • Cellular localization

      Membrane. Isoform 3 is targeted to the membrane via its interaction with RGS9BP.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • PERRS antibody
      • R9AP antibody
      • Regulator of G protein signalling 9 antibody
      • Regulator of G-protein signaling 9 antibody
      • RGS9 antibody
      • RGS9_HUMAN antibody
      • RGS9L antibody
      see all

    Images

    • Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
      Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
      All lanes : Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (purified)

      Lane 1 : Y79 whole cell lysate
      Lane 2 : Mouse spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 77 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?



      This data was developed using ab108975, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
      Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
      Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (purified) + PC-12 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 77 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?



      This data was developed using ab108975, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
      Immunocytochemistry - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
      This data was developed using ab108975, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling RGS9 with purified ab108975 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used. Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000). Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
    • Flow Cytometry - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
      Flow Cytometry - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
      This data was developed using ab108975, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of Y79 cells labelling RGS9 with purified ab108975 at a dilution of 1/60 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
    • Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
      Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
      All lanes : Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (unpurified)

      Lane 1 : PC12 lysates
      Lane 2 : Human spleen lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 77 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?



      This data was developed using ab108975, the same antibody clone in a different buffer formulation.

      Note: Predicted molecular weights for UniProt ID O75916 isoforms 1 to 4 are ~77, 55, 57 and 50 kDa respectively. Therefore, the observed WB band may correspond to isoform 2, 3 or 4.

    • Flow Cytometry - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
      Flow Cytometry - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
      This data was developed using ab108975, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized PC12 cells labelling RGS9 with unpurified ab108975 (red) at a dilution of 1/30 or a rabbit IgG (negative) (green).
    • Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)
      Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247728? Please let us know so that we can cite the reference in this datasheet.

    ab247728 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab247728.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com