Recombinant
RabMAb

Recombinant Anti-RGS9 antibody [EPR2873] - BSA and Azide free (ab247728)

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Overview

  • Product name

    Anti-RGS9 antibody [EPR2873] - BSA and Azide free
    See all RGS9 primary antibodies

  • Description

    Rabbit monoclonal [EPR2873] to RGS9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within RGS9. The exact sequence is proprietary.

  • General notes

    Ab247728 is the carrier-free version of ab108975. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247728 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab247728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 77 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction.

    • Tissue specificity

      Highly expressed in the caudate and putamen, lower levels found in the hypothalamus and nucleus accumbens and very low levels in cerebellum. Not expressed in globus pallidus or cingulate cortex. Isoform 2 is expressed predominantly in pineal gland and retina. Isoform 3 is expressed in retina (abundant in photoreceptors).

    • Involvement in disease

      Defects in RGS9 are a cause of prolonged electroretinal response suppression (PERRS) [MIM:608415]; also known as bradyopsia. PERRS is characterized by difficulty adjusting to sudden changes in luminance levels mediated by cones.

    • Sequence similarities

      Contains 1 DEP domain.
      Contains 1 G protein gamma domain.
      Contains 1 RGS domain.

    • Domain

      In photoreceptor cells the DEP domain is essential for targeting RGS9 to the outer rod segments.

    • Post-translational
      modifications

      Retinal isoform 3 is light-dependent phosphorylated at 'Ser-478'. Phosphorylation is decreased by light exposition.

    • Cellular localization

      Membrane. Isoform 3 is targeted to the membrane via its interaction with RGS9BP.
    • Information by UniProt

    • Database links

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    • Alternative names

      • PERRS antibody
      • R9AP antibody
      • Regulator of G protein signalling 9 antibody
      • Regulator of G-protein signaling 9 antibody
      • RGS9 antibody
      • RGS9_HUMAN antibody
      • RGS9L antibody
      see all

    References

    ab247728 has not yet been referenced specifically in any publications.

    Publishing research using ab247728? Please let us know so that we can cite the reference in this datasheet.

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