Overview

  • Product name
    Anti-RHAG antibody [EPR10011]
    See all RHAG primary antibodies
  • Description
    Rabbit monoclonal [EPR10011] to RHAG
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human RHAG (UniProt: Q02094).

  • Positive control
    • Human fetal kidney, fetal liver and bone marrow lysates; Human lung and spleen tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10011
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Relevance
      RHAG (Rhesus blood group-associated glycoprotein) is associated with rhesus blood group antigen expression. It may be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane. The Rh blood group antigens are associated with human erythrocyte membrane proteins of approximately 30 kD, the so-called Rh30 polypeptides. Heterogeneously glycosylated membrane proteins of 50 and 45 kD, the Rh50 glycoproteins, are coprecipitated with the Rh30 polypeptides on immunoprecipitation with anti-Rh-specific mono- and polyclonal antibodies. The Rh antigens appear to exist as a multisubunit complex of CD47, LW, glycophorin B and play a critical role in the Rh50 glycoprotein.
    • Cellular localization
      Cell Membrane; multi-pass membrane protein
    • Database links
    • Alternative names
      • Abnormal Rhesus blood group associated glycoprotein antibody
      • Ammonium transporter Rh type A antibody
      • CD241 antibody
      • CD241 antigen antibody
      • Erythrocyte membrane glycoprotein Rh50 antibody
      • Erythrocyte plasma membrane 50 kDa glycoprotein antibody
      • Rh associated glycoprotein antibody
      • Rh family type A glycoprotein antibody
      • Rh type A glycoprotein antibody
      • RH2 antibody
      • RH50 antibody
      • Rh50 glycoprotein antibody
      • Rh50 GP antibody
      • RH50A antibody
      • Rh50GP antibody
      • Rhesus associated polypeptide, 50-KD antibody
      • Rhesus blood group associated ammonia channel antibody
      • Rhesus blood group associated glycoprotein antibody
      • Rhesus blood group family type A glycoprotein antibody
      • SLC42A1 antibody
      see all

    Images

    • ab155094 showing +ve staining in Human normal tonsil.
    • ab155094 showing +ve staining in Human normal heart.
    • ab155094 showing +ve staining in Human normal colon.
    • All lanes : Anti-RHAG antibody [EPR10011] (ab155094) at 1/1000 dilution

      Lane 1 : Human fetal kidney cell lysate
      Lane 2 : Human fetal liver cell lysate
      Lane 3 : Human bone marrow cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 44 kDa

    • Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling RHAG with ab155094 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling RHAG with ab155094 at 1/250 dilution.

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab155094.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up