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    rhbdd1-antibody-ab220855.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-RHBDD1 antibody (ab220855)

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Immunocytochemistry/ Immunofluorescence - Anti-RHBDD1 antibody (ab220855)

    Key features and details

    • Rabbit polyclonal to RHBDD1
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-RHBDD1 antibody
      See all RHBDD1 primary antibodies
    • Description

      Rabbit polyclonal to RHBDD1
    • Host species

      Rabbit
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Orangutan
    • Immunogen

      Recombinant fragment corresponding to Human RHBDD1 aa 42-101.
      Sequence:

      FLNPQKPLYSSCLSVEKCYQQKDWQRLLLSPLHHADDWHLYFNMASMLWK GINLERRLGS


      Database link: Q8TEB9
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • U-2 OS cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Associated Proteins

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Our Abpromise guarantee covers the use of ab220855 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF Use a concentration of 0.25 - 2 µg/ml.

    Fixation/Permeabilization: PFA/Triton X-100.

    Target

    • Sequence similarities

      Belongs to the peptidase S54 family.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q8TEB9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 84236 Human
      • Entrez Gene: 76867 Mouse
      • Entrez Gene: 100172352 Orangutan
      • SwissProt: Q8TEB9 Human
      • SwissProt: Q8BHC7 Mouse
      • SwissProt: Q5RBS4 Orangutan
      • Unigene: 471514 Human
      • Unigene: 226994 Mouse
      • Alternative names

        • DKFZp547E052 antibody
        • EC=3.4.21. antibody
        • MGC117258 antibody
        • RHBD1_HUMAN antibody
        • RHBDD1 antibody
        • Rhomboid domain containing 1 antibody
        • Rhomboid domain-containing protein 1 antibody
        • Rhomboid like protein 4 antibody
        • RRP4 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-RHBDD1 antibody (ab220855)
        Immunocytochemistry/ Immunofluorescence - Anti-RHBDD1 antibody (ab220855)

        Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling RHBDD1 with ab220855 at 4 μg/ml (green).

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab220855? Please let us know so that we can cite the reference in this datasheet.

      ab220855 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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