Anti-RHBG antibody (ab106801)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-RHBG antibody
See all RHBG primary antibodies -
Description
Goat polyclonal to RHBG -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide:
PLRGGESDTR
by a Cysteine residue linker, corresponding to C terminal amino acids 445-454 of Mouse RHBG (NP_067350.2). -
Positive control
- Mouse Kidney lysates; lysates of cell lines NIH3T3 and NSO
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab106801 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab106801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa). |
Target
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Function
Functions as a specific ammonium transporter. -
Tissue specificity
Specifically expressed in kidney. Also detected in liver and ovary. -
Sequence similarities
Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily. -
Developmental stage
Fetally expressed by kidney and to a lower extent in liver. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Basolateral cell membrane. Cytoplasmic vesicle membrane. - Information by UniProt
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Database links
- Entrez Gene: 58176 Mouse
- SwissProt: Q8BUX5 Mouse
- Unigene: 103777 Mouse
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Alternative names
- Ammonium transporter Rh type B antibody
- Rh family B glycoprotein antibody
- Rh family type B glycoprotein antibody
see all
Images
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Anti-RHBG antibody (ab106801) at 1 µg/ml + NSO lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
This product has been referenced in:
- Takeda K & Takemasa T Expression of ammonia transporters Rhbg and Rhcg in mouse skeletal muscle and the effect of 6-week training on these proteins. Physiol Rep 3:N/A (2015). Read more (PubMed: 26471760) »