Key features and details
- Rabbit polyclonal to RHCG
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RHCG antibody
See all RHCG primary antibodies
DescriptionRabbit polyclonal to RHCG
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- Human tonsil tissue lysate, Human esophagus tissue
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab187904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 53 kDa.|
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFunctions as an electroneutral and bidirectional ammonium transporter. May regulate transepithelial ammonia secretion.
Tissue specificityExpressed in brain, testis, placenta, pancreas, esophagus and prostate. Expressed in squamous epithelial tissues (at protein level). According to PubMed:11062476, specifically expressed in kidney.
Sequence similaritiesBelongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.
Developmental stageSpecifically expressed in fetal kidney.
Cellular localizationApical cell membrane. Also detected at the basolateral membrane and in subapical vesicles.
- Information by UniProt
- Ammonium transporter Rh type C antibody
- C15orf6 antibody
- CDRC2 antibody
All lanes : Anti-RHCG antibody (ab187904) at 1/100 dilution
Lane 1 : Human cell line RT-4 lysate
Lane 2 : Human cell line U-251MG sp lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 1 minute
Immunohistochemical analysis of paraffin-embedded Human esophagus tissue labeling RHCG with ab187904 at 1/1000 dilution.
ab187904 has not yet been referenced specifically in any publications.