Product nameAnti-RHEB antibody
See all RHEB primary antibodies
DescriptionRabbit polyclonal to RHEB
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla
Synthetic peptide corresponding to Human RHEB aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: Raji; SHSY5Y; EGF treated A431; Jurkat.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab109914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).|
FunctionStimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
Tissue specificityUbiquitous. Highest levels observed in skeletal and cardiac muscle.
Sequence similaritiesBelongs to the small GTPase superfamily. Rheb family.
modificationsFarnesylation is important for efficiently activating mTORC1-mediated signaling.
Cellular localizationCell membrane.
- Information by UniProt
- Ras homolog enriched in brain 2, formerly antibody
- GTP binding protein Rheb antibody
- GTP-binding protein Rheb antibody
All lanes : Anti-RHEB antibody (ab109914) at 1 µg/ml
Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : EGF-Stimulated A431 Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa, 49 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab109914 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab109914 has not yet been referenced specifically in any publications.