Anti-RHEB antibody (ab25873)
Key features and details
- Rabbit polyclonal to RHEB
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-RHEB antibody
See all RHEB primary antibodies -
Description
Rabbit polyclonal to RHEB -
Host species
Rabbit -
Specificity
ab25873 recognises Rheb (Ras homolog enriched in brain). -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Drosophila melanogaster -
Immunogen
A 15 amino acid peptide from the middle region of Human Rheb.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 2 µg/ml.
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ICC/IF |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
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Notes |
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IHC-P
Use a concentration of 2 µg/ml. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. |
Target
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Function
Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity. -
Tissue specificity
Ubiquitous. Highest levels observed in skeletal and cardiac muscle. -
Sequence similarities
Belongs to the small GTPase superfamily. Rheb family. -
Post-translational
modificationsFarnesylation is important for efficiently activating mTORC1-mediated signaling. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 6009 Human
- Entrez Gene: 19744 Mouse
- Entrez Gene: 26954 Rat
- Omim: 601293 Human
- SwissProt: Q15382 Human
- SwissProt: Q921J2 Mouse
- SwissProt: Q62639 Rat
- Unigene: 283521 Human
see all -
Alternative names
- Ras homolog enriched in brain 2, formerly antibody
- GTP binding protein Rheb antibody
- GTP-binding protein Rheb antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab25873 has been referenced in 17 publications.
- Sun X et al. Mechanistic target of rapamycin complex 1 orchestrates the interplay between hepatocytes and Kupffer cells to determine the outcome of immune-mediated hepatitis. Cell Death Dis 13:1031 (2022). PubMed: 36494334
- Lu Q et al. Complement factor B in high glucose-induced podocyte injury and diabetic kidney disease. JCI Insight 6:N/A (2021). PubMed: 34622800
- Li Z et al. miR-155-5p upregulation ameliorates myocardial insulin resistance via mTOR signaling in chronic alcohol drinking rats. PeerJ 9:e10920 (2021). PubMed: 33868799
- Tang L et al. Electroacupuncture Upregulated Ghrelin in Rats with Functional Dyspepsia via AMPK/TSC2/Rheb-Mediated mTOR Inhibition. Dig Dis Sci 65:1689-1699 (2020). PubMed: 31863340
- Li Z et al. miR-155-5p alleviates ethanol-induced myocardial insulin resistance in H9C2 cells via regulating the mTOR signalling pathway. Mol Biol Rep 47:9469-9477 (2020). PubMed: 33159675