Overview

  • Product name

    Anti-Rheumatoid Factor IgM ELISA Kit
  • Detection method

    Colorimetric
  • Sample type

    Serum, Heparin Plasma, Citrate Plasma
  • Assay type

    Indirect
  • Sensitivity

    < 0.5 U/ml
  • Assay duration

    Multiple steps standard assay
  • Product overview

    Abcam’s anti-Rheumatoid Factor IgM ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to Rheumatoid Factor IgM in Human serum or plasma.

    The quantitative immunoenzymatic determination of IgM rheumatoid factors is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strips are precoated with Fc fragments of Human immunoglobulin G to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added. This conjugate binds to the captured rheumatoid factors. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of IgM rheumatoid factors in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

  • Tested applications

    Suitable for: Indirect ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab178653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Images

Protocols

References

This product has been referenced in:

  • Qian X  et al. Serum proteomic analysis of the anti-arthritic effects of sinomenine on rats with collagen-induced arthritis. Mol Med Rep 18:49-58 (2018). Read more (PubMed: 29749546) »
See 1 Publication for this product

Customer reviews and Q&As

Senior Scientist

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The anti-rheumatoid factor IgM ELISA kit is easy to use as the standards and controls are supplied ready for use. The incubation times are short (30 minutes) allowing for quick results in less than 2 hours. My results have been consistent and reliable.

Abcam user community

Verified customer

Submitted Sep 04 2019

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