Recombinant full length protein corresponding to Human Rho A + B + C aa 1-193.
MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDG KQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWT PEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANR IGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL
Our Abpromise guarantee covers the use of ab175328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1500.|
|IP||Use at 2 µg/mg of lysate.|
|ICC/IF||1/100 - 1/200.|
Immunofluorescence analysis of formalin-fixed permeabilized mouse NIH 3T3 cells, labeling Rho A + B + C (green, left panel) using ab175328 at a 1/100 dilution followed by DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye (central panel).
Western blot analysis on immunoprecipitation pellet from HeLa cells. The antigen-antibody complex was formed by incubating 750 µg of HeLa cell lysate with 2 µg of ab175328 overnight at 4°C. The immune-complex was then captured on 50 µl of Protein A/G Plus Agarose, washed extensively and eluted in sample buffer. 1) 25 µg of HeLa cell lysate and 2) eluted sample were resolved on a SDS PAGE gel. The membrane was probed with ab175328 at a 1/1000 dilution. An anti-mouse IgG-HRP secondary antibody at a 1/2500 dilution was used. Chemiluminescent detection was perfomed.
ab175328 has not yet been referenced specifically in any publications.
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