Key features and details
- Mouse monoclonal [1A11-4G10] to Rho A + B + C
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgG1
Product nameAnti-Rho A + B + C antibody [1A11-4G10]
See all Rho A + B + C primary antibodies
DescriptionMouse monoclonal [1A11-4G10] to Rho A + B + C
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Mouse
Predicted to work with: Chicken, Cow, Dog, Caenorhabditis elegans, Drosophila melanogaster, Orangutan
Recombinant full length protein corresponding to Human Rho A + B + C aa 1-193.
MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDG KQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWT PEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANR IGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL
Database link: P61586
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol (glycerin, glycerine), 69% PBS
Concentration information loading...
PurityProtein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/100.
1/10 - 1/100.
Cellular localizationRho A: Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm; cytoskeleton. Rho B: Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Rho C: Cell membrane; Lipid-anchor; Cytoplasmic side.
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Immunofluorescence analysis of formalin-fixed permeabilized mouse kidney tissue (right) compared to a negative control without primary antibody (left), labeling Rho A + B + C (green, left panel) using ab175328 at a 1/120 dilution followed by DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye.
ab175328 has not yet been referenced specifically in any publications.