Product nameAnti-Rho A + B + C antibody [1A11-4G10]
See all Rho A + B + C primary antibodies
DescriptionMouse monoclonal [1A11-4G10] to Rho A + B + C
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Dog, Caenorhabditis elegans, Drosophila melanogaster, Orangutan
Recombinant full length protein corresponding to Human Rho A + B + C aa 1-193.
MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDG KQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWT PEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANR IGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL
Database link: P61586
- HeLa, A431, HEK293T, U2OS and mouse NIH 3T3 cell lysates; mouse NIH 3T3 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol, 69% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab175328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1500.|
|IP||Use at 2 µg/mg of lysate.|
|ICC/IF||1/100 - 1/200.|
Cellular localizationRho A: Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm; cytoskeleton. Rho B: Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Rho C: Cell membrane; Lipid-anchor; Cytoplasmic side.
- AA017882 antibody
- AI324259, antibody
- Aplysia ras related homolog 9 (RhoC) antibody
All lanes : Anti-Rho A + B + C antibody [1A11-4G10] (ab175328) at 1/1000 dilution
Lane 1 : HeLa cell lysate at 25 µg
Lane 2 : A431 cell lysate at 25 µg
Lane 3 : HEK293T cell lysate at 25 µg
Lane 4 : U2OS cell lysate at 25 µg
Lane 5 : Mouse NIH 3T3 cell lysate at 25 µg
Lane 6 : Purified full length His-tagged Rho A at 0.5 µg
Lane 7 : Purified truncated Rho B at 0.5 µg
Lane 8 : Purified full length Rho C at 0.5 µg
All lanes : Goat anti-mouse IgG-HRP at 1/15000 dilution
Developed using the ECL technique.
Immunofluorescence analysis of formalin-fixed permeabilized mouse NIH 3T3 cells, labeling Rho A + B + C (green, left panel) using ab175328 at a 1/100 dilution followed by DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye (central panel).
Western blot analysis on immunoprecipitation pellet from HeLa cells. The antigen-antibody complex was formed by incubating 750 µg of HeLa cell lysate with 2 µg of ab175328 overnight at 4°C. The immune-complex was then captured on 50 µl of Protein A/G Plus Agarose, washed extensively and eluted in sample buffer. 1) 25 µg of HeLa cell lysate and 2) eluted sample were resolved on a SDS PAGE gel. The membrane was probed with ab175328 at a 1/1000 dilution. An anti-mouse IgG-HRP secondary antibody at a 1/2500 dilution was used. Chemiluminescent detection was perfomed.
ab175328 has not yet been referenced specifically in any publications.