Overview

  • Product name

    Anti-Rho antibody [Y486]
    See all Rho primary antibodies
  • Description

    Rabbit monoclonal [Y486] to Rho
  • Host species

    Rabbit
  • Specificity

    The antibody is predicted to detect RhoA, RhoB and RhoC based on sequence analysis.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Rho aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • PC12 cell lysate and hepatocellular carcinoma.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    Y486
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
    • Sequence similarities

      Belongs to the small GTPase superfamily. Rho family.
    • Domain

      The basic-rich region is essential for yopT recognition and cleavage.
    • Post-translational
      modifications

      Substrate for botulinum ADP-ribosyltransferase.
      Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
      AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
      Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling.
      Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
    • Cellular localization

      Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Cytoplasm > cell cortex. Midbody. Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner.
    • Information by UniProt
    • Database links

    • Alternative names

      • ARH12 antibody
      • ARHA antibody
      • H12 antibody
      • ras homolog gene family member A antibody
      • ras homolog gene family member B antibody
      • ras homolog gene family member C antibody
      • Rho cDNA clone 12 antibody
      • RHO12 antibody
      • RHOA antibody
      • RHOA_HUMAN antibody
      • rhob antibody
      • rhoc antibody
      • RHOH12 antibody
      • Small GTP binding protein RhoA antibody
      • Transforming protein RhoA antibody
      see all

    Images

    • Anti-Rho antibody [Y486] (ab32046) at 1/2000 dilution + PC12 cell lysate

      Predicted band size: 21 kDa
      Observed band size: 22 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab32046 at 1/100 dilution.

    References

    This product has been referenced in:

    • Soon PS  et al. Profiling differential microRNA expression between in situ, infiltrative and lympho-vascular space invasive breast cancer: a pilot study. Clin Exp Metastasis 35:3-13 (2018). Read more (PubMed: 29214365) »
    • Garcia-Vidal E  et al. Evaluation of the Innate Immune Modulator Acitretin as a Strategy To Clear the HIV Reservoir. Antimicrob Agents Chemother 61:N/A (2017). Read more (PubMed: 28874382) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (lung epithelial cells)
    Specification
    lung epithelial cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.2% saponin
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Oct 31 2012

    Answer

    Thank you for your enquiry. I have been in touch with the lab for their comments. Unfortunately this has not been tested. From a literature search I can see that there are 3 binding sites for GTP, one of which overlaps with the tail end of the N-term antibody binding region. Given that there is about 3 amino acid overlap this could potentially disrupt binding following a conformational change induced by the binding of dTTP or dGTP. I am sorry that I cannot be more definitive in my answer.

    Read More

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