Key features and details
- Rabbit polyclonal to Rho guanine exchange factor 16/NBR
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Rho guanine exchange factor 16/NBR antibody
See all Rho guanine exchange factor 16/NBR primary antibodies
DescriptionRabbit polyclonal to Rho guanine exchange factor 16/NBR
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to Human Rho guanine exchange factor 16/NBR aa 125-175.
Database link: NP_055263.2
- Hela or 293T cell lysate
This product was previously labelled as Rho guanine exchange factor 16
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 80 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionGuanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.
DomainThe PDZ-binding motif mediates interaction with TAX1BP3.
- Information by UniProt
- ARHGEF 16 antibody
- ARHGEF16 antibody
- ARHGG_HUMAN antibody
All lanes : Anti-Rho guanine exchange factor 16/NBR antibody (ab86068) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 80 kDa
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Rho guanine exchange factor 16/NBR with ab86068 at 1/200 (1µg/ml). Detection: DAB.
Detection of Rho guanine exchange factor 16/NBR by Western blot of Immunprecipitate.
ab86068, at 1 µg/ml, staining Rho guanine exchange factor 16/NBR in HeLa whole cell lysate immunoprecipitated using ab86068 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 3 seconds.
ab86068 has been referenced in 1 publication.
- Huang D et al. GLI2 promotes cell proliferation and migration through transcriptional activation of ARHGEF16 in human glioma cells. J Exp Clin Cancer Res 37:247 (2018). PubMed: 30305138