Overview

  • Product name

    Anti-Rho guanine exchange factor 16/NBR antibody
    See all Rho guanine exchange factor 16/NBR primary antibodies
  • Description

    Rabbit polyclonal to Rho guanine exchange factor 16/NBR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Rho guanine exchange factor 16/NBR aa 125-175.
    Database link: NP_055263.2

  • Positive control

    • Hela or 293T cell lysate
  • General notes

     This product was previously labelled as Rho guanine exchange factor 16

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 80 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6.
  • Sequence similarities

    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Domain

    The PDZ-binding motif mediates interaction with TAX1BP3.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARHGEF 16 antibody
    • ARHGEF16 antibody
    • ARHGG_HUMAN antibody
    • Ephexin 4 antibody
    • Ephexin-4 antibody
    • EPHEXIN4 antibody
    • GEF16 antibody
    • NBR antibody
    • Putative Neuroblastoma Protein antibody
    • Rho guanine exchange factor (GEF) 16 antibody
    • Rho guanine nucleotide exchange factor (GEF) 16 antibody
    • Rho guanine nucleotide exchange factor 16 antibody
    see all

Images

  • All lanes : Anti-Rho guanine exchange factor 16/NBR antibody (ab86068) at 0.04 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 80 kDa


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Rho guanine exchange factor 16/NBR with ab86068 at 1/200 (1µg/ml). Detection: DAB.

  • Detection of Rho guanine exchange factor 16/NBR by Western blot of Immunprecipitate.
    ab86068, at 1 µg/ml, staining Rho guanine exchange factor 16/NBR in HeLa whole cell lysate immunoprecipitated using ab86068 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 3 seconds.

References

This product has been referenced in:

  • Huang D  et al. GLI2 promotes cell proliferation and migration through transcriptional activation of ARHGEF16 in human glioma cells. J Exp Clin Cancer Res 37:247 (2018). Read more (PubMed: 30305138) »
See 1 Publication for this product

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