RecombinantRabMAb

Recombinant Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

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Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
  • Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
  • Immunocytochemistry/ Immunofluorescence - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
  • Flow Cytometry (Intracellular) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
  • Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9950] to Rho guanine exchange factor 16/NBR
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950]
    See all Rho guanine exchange factor 16/NBR primary antibodies

  • Description

    Rabbit monoclonal [EPR9950] to Rho guanine exchange factor 16/NBR

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, MCF-7 and 293T cell lysates; Mouse kidney lysates; Human cervix carcinoma tissue; Human breast tissue; HeLa cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     This product was previously labelled as Rho guanine exchange factor 16

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9950

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6.

    • Sequence similarities

      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 SH3 domain.

    • Domain

      The PDZ-binding motif mediates interaction with TAX1BP3.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • ARHGEF 16 antibody
        • ARHGEF16 antibody
        • ARHGG_HUMAN antibody
        • Ephexin 4 antibody
        • Ephexin-4 antibody
        • EPHEXIN4 antibody
        • GEF16 antibody
        • NBR antibody
        • Putative Neuroblastoma Protein antibody
        • Rho guanine exchange factor (GEF) 16 antibody
        • Rho guanine nucleotide exchange factor (GEF) 16 antibody
        • Rho guanine nucleotide exchange factor 16 antibody
        see all

      Images

      • Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
        Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
        Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785) at 1/1000 dilution + Mouse kidney lysate at 20 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated)

        Predicted band size: 80 kDa
        Observed band size: 80 kDa


        Exposure time: 180 seconds


        Blocking and Diluting buffer: 5% NFDM/TBST

      • Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
        Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
        All lanes : Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : MCF-7 cell lysate
        Lane 3 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 80 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

        Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

        Immunohistochemical analysis of paraffin-embedded Human normal breast tissue labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
        Immunocytochemistry/ Immunofluorescence - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

        Immunofluorescent analysis of HeLa cells labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/100 dilution.

      • Flow Cytometry (Intracellular) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
        Flow Cytometry (Intracellular) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

        Flow cytometric analysis of permeabilized HeLa cells labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

        Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154785 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

        Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154785 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

        Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154785 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

        Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154785 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
        Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

      References (0)

      Publishing research using ab154785? Please let us know so that we can cite the reference in this datasheet.

      ab154785 has not yet been referenced specifically in any publications.

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