Recombinant Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9950] to Rho guanine exchange factor 16/NBR
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950]
See all Rho guanine exchange factor 16/NBR primary antibodies -
Description
Rabbit monoclonal [EPR9950] to Rho guanine exchange factor 16/NBR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
corresponding to Human Rho guanine exchange factor 16/NBR.
Database link: Q5VV41 -
Positive control
- HeLa, MCF-7 and 293T cell lysates; Mouse kidney lysates; Human cervix carcinoma tissue; Human breast tissue; HeLa cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as Rho guanine exchange factor 16
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9950 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 80 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6. -
Sequence similarities
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain. -
Domain
The PDZ-binding motif mediates interaction with TAX1BP3. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 27237 Human
- Entrez Gene: 230972 Mouse
- SwissProt: Q5VV41 Human
- SwissProt: Q3U5C8 Mouse
- Unigene: 87435 Human
- Unigene: 38481 Mouse
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Alternative names
- ARHGEF 16 antibody
- ARHGEF16 antibody
- ARHGG_HUMAN antibody
see all
Images
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Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785) at 1/1000 dilution + Mouse kidney lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated)
Predicted band size: 80 kDa
Observed band size: 80 kDa
Exposure time: 180 secondsBlocking and Diluting buffer: 5% NFDM/TBST
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All lanes : Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 80 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
Immunohistochemical analysis of paraffin-embedded Human normal breast tissue labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
Immunofluorescent analysis of HeLa cells labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/100 dilution.
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Flow cytometric analysis of permeabilized HeLa cells labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154785 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154785 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154785 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154785 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154785 has not yet been referenced specifically in any publications.