Recombinant Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
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Overview
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Product nameAnti-Rho guanine exchange factor 16/NBR antibody [EPR9950]
See all Rho guanine exchange factor 16/NBR primary antibodies -
DescriptionRabbit monoclonal [EPR9950] to Rho guanine exchange factor 16/NBR
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP -
Species reactivityReacts with: Mouse, Human
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Immunogen
corresponding to Human Rho guanine exchange factor 16/NBR.
Database link: Q5VV41 -
Positive control
- HeLa, MCF-7 and 293T cell lysates; Human cervix carcinoma tissue; Human breast tissue; HeLa cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as Rho guanine exchange factor 16
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR9950
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 80 kDa. | |
| IHC-P | 1/50 - 1/100. | |
| ICC/IF | 1/100 - 1/250. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionGuanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6.
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Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain. -
DomainThe PDZ-binding motif mediates interaction with TAX1BP3.
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Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 27237 Human
- Entrez Gene: 230972 Mouse
- SwissProt: Q5VV41 Human
- SwissProt: Q3U5C8 Mouse
- Unigene: 87435 Human
- Unigene: 38481 Mouse
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Alternative names
- ARHGEF 16 antibody
- ARHGEF16 antibody
- ARHGG_HUMAN antibody
see all
Images
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![Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)](https://www.abcam.com/ps/products/154/ab154785/Images/Rho-guanine-exchange-factor-16-Primary-antibodies-ab154785-6.jpg)
Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)All lanes : Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 80 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)](https://www.abcam.com/ps/products/154/ab154785/Images/Rho-guanine-exchange-factor-16-Primary-antibodies-ab154785-2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/50 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)](https://www.abcam.com/ps/products/154/ab154785/Images/Rho-guanine-exchange-factor-16-Primary-antibodies-ab154785-3.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)Immunohistochemical analysis of paraffin-embedded Human normal breast tissue labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/50 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)](https://www.abcam.com/ps/products/154/ab154785/Images/Rho-guanine-exchange-factor-16-Primary-antibodies-ab154785-4.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)Immunofluorescent analysis of HeLa cells labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/100 dilution.
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![Flow Cytometry - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)](https://www.abcam.com/ps/products/154/ab154785/Images/Rho-guanine-exchange-factor-16-Primary-antibodies-ab154785-7.jpg)
Flow Cytometry - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)Flow cytometric analysis of permeabilized HeLa cells labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)](https://www.abcam.com/ps/products/154/ab154785/Images/Rho-guanine-exchange-factor-16-Primary-antibodies-ab154785-8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154785 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)](https://www.abcam.com/ps/products/154/ab154785/Images/Rho-guanine-exchange-factor-16-Primary-antibodies-ab154785-9.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154785 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)](https://www.abcam.com/ps/products/154/ab154785/Images/Rho-guanine-exchange-factor-16-Primary-antibodies-ab154785-10.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154785 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)](https://www.abcam.com/ps/products/154/ab154785/Images/Rho-guanine-exchange-factor-16-Primary-antibodies-ab154785-11.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154785 showing +ve staining.
Protocols
Datasheets and documents
References
ab154785 has not yet been referenced specifically in any publications.
