Recombinant
RabMAb

Recombinant Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

Overview

  • Product name
    Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950]
    See all Rho guanine exchange factor 16/NBR primary antibodies
  • Description
    Rabbit monoclonal [EPR9950] to Rho guanine exchange factor 16/NBR
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    corresponding to Human Rho guanine exchange factor 16/NBR.
    Database link: Q5VV41

  • Positive control
    • HeLa, MCF-7 and 293T cell lysates; Human cervix carcinoma tissue; Human breast tissue; HeLa cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     This product was previously labelled as Rho guanine exchange factor 16

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6.
    • Sequence similarities
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 SH3 domain.
    • Domain
      The PDZ-binding motif mediates interaction with TAX1BP3.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARHGEF 16 antibody
      • ARHGEF16 antibody
      • ARHGG_HUMAN antibody
      • Ephexin 4 antibody
      • Ephexin-4 antibody
      • EPHEXIN4 antibody
      • GEF16 antibody
      • NBR antibody
      • Putative Neuroblastoma Protein antibody
      • Rho guanine exchange factor (GEF) 16 antibody
      • Rho guanine nucleotide exchange factor (GEF) 16 antibody
      • Rho guanine nucleotide exchange factor 16 antibody
      see all

    Images

    • All lanes : Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 80 kDa

    • Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human normal breast tissue labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/50 dilution.

    • Immunofluorescent analysis of HeLa cells labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/100 dilution.

    • Flow cytometric analysis of permeabilized HeLa cells labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    • Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154785 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154785 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154785 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154785 showing +ve staining.

    References

    ab154785 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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