Product nameRhoB Activation Assay Kit
Sample typeTissue, Adherent cells, Suspension cells
Species reactivityReacts with: Mouse, Rat, Human
RhoB Activation Assay Kit (ab211165) uses Rhotekin RBD Agarose beads to selectively isolate and pull-down the active form of Rho from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rho is detected by western blot analysis using an anti-RhoB specific polyclonal antibody, which detects the human, mouse and rat protein.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
RhoB is a 21 kDa protein that regulates a variety of biological response pathways that include cell growth, cell transformation and tumor invasion. Like other small GTPases, RhoB cycles between an inactive GDP-bound form and an active GTP-bound form: in its active (GTP-bound) state, RhoB binds specifically to the Rho-binding domain (RBD) of Rhotekin to control downstream signaling cascades.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 20 tests 5 tests ab211178 - Rhotekin RBD Agarose Beads 1 x 800µl 1 x 200µl 100X GTPyS 1 x 50µl 1 x 20µl 100X GDP 1 x 50µl 1 x 20µl 5X Assay/Lysis Buffer 1 x 30ml 4 x 2ml Anti-rhoB Rabbit polyclonal antibody 1 x 40µl 1 x 10µl RhoB immunoblot Positive Control 1 x 100µl 1 x 100µl
FunctionMediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.
Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
modificationsPrenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome.
Cellular localizationLate endosome membrane. Cell membrane. Nucleus. Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.
- Information by UniProt
- Aplysia RAS-related homolog 6
ab211165 has not yet been referenced specifically in any publications.