Overview

  • Product name

    RhoB Activation Assay Kit
  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Product overview

    RhoB Activation Assay Kit (ab211165) uses Rhotekin RBD Agarose beads to selectively isolate and pull-down the active form of Rho from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rho is detected by western blot analysis using an anti-RhoB specific polyclonal antibody, which detects the human, mouse and rat protein.


    Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.

  • Notes

    Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.

    RhoB is a 21 kDa protein that regulates a variety of biological response pathways that include cell growth, cell transformation and tumor invasion. Like other small GTPases, RhoB cycles between an inactive GDP-bound form and an active GTP-bound form: in its active (GTP-bound) state, RhoB binds specifically to the Rho-binding domain (RBD) of Rhotekin to control downstream signaling cascades.

Properties

Associated products

Images

  • RhoB positive control (recombinant human RhoB from E. coli, partially purified) detected by immunoblot.

  • Dot blot that demonstrates specificity of anti-RhoB rabbit antibody.

Protocols

References

ab211165 has not yet been referenced specifically in any publications.

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