Anti-RHOB antibody [2916C2a] (ab53743)
Key features and details
- Mouse monoclonal [2916C2a] to RHOB
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-RHOB antibody [2916C2a]
See all RHOB primary antibodies -
Description
Mouse monoclonal [2916C2a] to RHOB -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment (Human)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from culture supernatant -
Clonality
Monoclonal -
Clone number
2916C2a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
Target
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Function
Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Post-translational
modificationsPrenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome. -
Cellular localization
Late endosome membrane. Cell membrane. Nucleus. Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. - Information by UniProt
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Alternative names
- Aplysia RAS-related homolog 6 antibody
- ARH6 antibody
- ARHB antibody
see all
Images
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Anti-RHOB antibody [2916C2a] (ab53743) + immunized recombinant protein
Predicted band size: 22 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
The band on the Wetstern blot does not correspond to the predicted size, as the recombinant protein was used as the test antigen
Datasheets and documents
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Datasheet download
References (4)
ab53743 has been referenced in 4 publications.
- Yao X et al. LncRNA GAS5 Regulates Osteosarcoma Cell Proliferation, Migration, and Invasion by Regulating RHOB via Sponging miR-663a. Cancer Manag Res 12:8253-8261 (2020). PubMed: 32982421
- Liu H et al. Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE. Exp Ther Med 18:5072-5077 (2019). PubMed: 31819770
- Quinn K et al. Rho GTPases modulate entry of Ebola virus and vesicular stomatitis virus pseudotyped vectors. J Virol 83:10176-86 (2009). PubMed: 19625394
- Murray D et al. NET1-mediated RhoA activation facilitates lysophosphatidic acid-induced cell migration and invasion in gastric cancer. Br J Cancer 99:1322-9 (2008). WB ; Human . PubMed: 18827818