- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-RHOB antibody
See all RHOB primary antibodies -
DescriptionRabbit polyclonal to RHOB
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IHC-Pmore details
-
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide within Human RHOB aa 103-137 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P62745 -
Positive control
- HL60 cell lysate; Human brain tissue.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityAmmonium Sulphate Precipitation
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50 - 1/100. Predicted molecular weight: 22 kDa. | |
| IHC-P | 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
FunctionMediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.
-
Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
-
Post-translational
modificationsPrenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome. -
Cellular localizationLate endosome membrane. Cell membrane. Nucleus. Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.
- Information by UniProt
-
Database links
- Entrez Gene: 388 Human
- Omim: 165370 Human
- SwissProt: P62745 Human
- Unigene: 502876 Human
-
Alternative names
- Aplysia RAS-related homolog 6 antibody
- ARH6 antibody
- ARHB antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHOB antibody (ab170611)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling RHOB with ab170611 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
-
Western blot - Anti-RHOB antibody (ab170611)Anti-RHOB antibody (ab170611) at 1/50 dilution + HL60 cell lysate at 35 µg
Predicted band size: 22 kDa
References
ab170611 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"