Anti-RHOB antibody (ab170611)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RHOB antibody
See all RHOB primary antibodies -
Description
Rabbit polyclonal to RHOB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human RHOB aa 103-137 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P62745 -
Positive control
- HL60 cell lysate; Human brain tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab170611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50 - 1/100. Predicted molecular weight: 22 kDa. | |
| IHC-P | 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Post-translational
modificationsPrenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome. -
Cellular localization
Late endosome membrane. Cell membrane. Nucleus. Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 388 Human
- Omim: 165370 Human
- SwissProt: P62745 Human
- Unigene: 502876 Human
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Alternative names
- Aplysia RAS-related homolog 6 antibody
- ARH6 antibody
- ARHB antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHOB antibody (ab170611)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling RHOB with ab170611 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot - Anti-RHOB antibody (ab170611)Anti-RHOB antibody (ab170611) at 1/50 dilution + HL60 cell lysate at 35 µg
Predicted band size: 22 kDa
Datasheets and documents
References
ab170611 has not yet been referenced specifically in any publications.
