Overview

  • Product name
  • Description
    Rabbit polyclonal to RHOB
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RHOB aa 103-137 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P62745

  • Positive control
    • HL60 cell lysate; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab170611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 22 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rho family.
  • Post-translational
    modifications
    Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome.
  • Cellular localization
    Late endosome membrane. Cell membrane. Nucleus. Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aplysia RAS-related homolog 6 antibody
    • ARH6 antibody
    • ARHB antibody
    • H6 antibody
    • MST081 antibody
    • MSTP081 antibody
    • oncogene RHO H6 antibody
    • ras homolog family member B antibody
    • ras homolog gene family, member B antibody
    • Rho cDNA clone 6 antibody
    • Rho related GTP binding protein RhoB Precursor antibody
    • Rho-related GTP-binding protein RhoB antibody
    • Rhob antibody
    • RHOB_HUMAN antibody
    • RHOH6 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling RHOB with ab170611 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-RHOB antibody (ab170611) at 1/50 dilution + HL60 cell lysate at 35 µg

    Predicted band size: 22 kDa

References

ab170611 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170611.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up