Anti-RHOC antibody (ab180785)
Key features and details
- Rabbit polyclonal to RHOC
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-RHOC antibody
See all RHOC primary antibodies -
Description
Rabbit polyclonal to RHOC -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Cow -
Immunogen
Recombinant fragment corresponding to Human RHOC aa 30-193.
Sequence:FPEVYVPTVFE NYIADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD LRQDEHTRRE LAKMKQEPVR SEEGRDMANR ISAFGYLECS AKTKEGVREV FEMATRAGLQ VRKNKRRRGC PIL
Database link: P08134 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Predicted molecular weight: 22 kDa.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Predicted molecular weight: 22 kDa. |
Target
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Function
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 389 Human
- Entrez Gene: 11853 Mouse
- Omim: 165380 Human
- SwissProt: P08134 Human
- SwissProt: Q62159 Mouse
- Unigene: 502659 Human
- Unigene: 658289 Human
- Unigene: 262 Mouse
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Alternative names
- Aplysia RAS-related homolog 9 antibody
- ARH 9 antibody
- ARH9 antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling RHOC with ab180785 (shown in green) at a dilution of 1/50. Nuclear DNA was labelled with DAPI (shown in blue).
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All lanes : Anti-RHOC antibody (ab180785) at 1/500 dilution
Lane 1 : A549 cell lysate
Lane 2 : NIH 3T3 cell lysate
Predicted band size: 22 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab180785 has been referenced in 5 publications.
- Yin P et al. Chemoprevention of 4NQO-Induced Mouse Tongue Carcinogenesis by AKT Inhibitor through the MMP-9/RhoC Signaling Pathway and Autophagy. Anal Cell Pathol (Amst) 2022:3770715 (2022). PubMed: 36247874
- Gao F et al. Knockdown of RhoC Inhibits Oral Squamous Cell Carcinoma Cell Invasion and Metastasis via Regulation of HMGA2. J Oncol 2021:6644077 (2021). PubMed: 33519932
- Yin P et al. MMP-9 Knockdown Inhibits Oral Squamous Cell Carcinoma Lymph Node Metastasis in the Nude Mouse Tongue-Xenografted Model through the RhoC/Src Pathway. Anal Cell Pathol (Amst) 2021:6683391 (2021). PubMed: 33828938
- Li J et al. WJ-MSCs intervention may relieve intrauterine adhesions in female rats via TGF-β1-mediated Rho/ROCK signaling inhibition. Mol Med Rep 23:N/A (2021). PubMed: 33179074
- Jin F et al. The pathophysiological nature of sarcomeres in trigger points in patients with myofascial pain syndrome: A preliminary study. Eur J Pain 24:1968-1978 (2020). PubMed: 32841448