Overview

  • Product name

    Rhod-2 AM, fluorescent Ca2+ indicator
  • Description

    Cell-permeable fluorescent Ca2+ indicator
  • Purity

    > 93%
  • CAS Number

    145037-81-6
  • Chemical structure

    Chemical Structure

Properties

References

This product has been referenced in:

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
SH-SY5Y cells were grown overnight on coverslips treated with histogrip. Rhod-2AM was prepared in serum free DMEM-F12 media to a final concentration of 5 μm and used to treat the cells for 30 minutes. Negative controls were treated with serum free DMEM-F12 media without Rhod-2AM. Following treatment, cells were washed gently in PBS 3 times and fixed with 4% PFA for 10 minutes. Cells were washed and stained with DAPI for 2 minutes, and mounted on slides using Antifade fluorescence mounting media.

Abcam user community

Verified customer

Submitted Feb 28 2018

Abreviews
SH-SY5Y cells were grown overnight on coverslips treated with histogrip. Rhod-2AM was prepared in serum free DMEM-F12 media to a final concentration of 5 μm and used to treat the cells for 30 minutes. Negative controls were treated with serum free DMEM-F12 media without Rhod-2AM. Following treatment, cells were washed gently in PBS 3 times and fixed with 4% PFA for 10 minutes. Cells were then stained with DAPI for 2 minutes, and mounted on slides using Antifade fluorescence mounting media.

Anu Shanu

Verified customer

Submitted Feb 27 2018

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