Product nameRhodamine Phalloidin Reagent
See all F-actin kits
Sample typeAdherent cells, Suspension cells
Assay typeCell-based (qualitative)
Rhodamine Phalloidin Reagent ab235138 is one of a series of phalloidin conjugates that are used to stain actin filaments in fluoresence microscopy.
Phalloidin conjugates are convenient probes for labeling, identifying and quantitating animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
Review our other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 647, Phalloidin-iFluor 594, and Phalloidin-iFluor 555, or search our website to see the rest of the range.
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 300 tests Rhodamine Phalloidin Conjugate 1 x 300 tests
This product has been referenced in:
- Fei WY et al. Magnolol prevents ovariectomy-induced bone loss by suppressing osteoclastogenesis via inhibition of the nuclear factor-?B and mitogen-activated protein kinase pathways. Int J Mol Med 43:1669-1678 (2019). Read more (PubMed: 30816431) »