Product nameAnti-Rhodopsin antibody [4D2] (FITC)
See all Rhodopsin primary antibodies
DescriptionMouse monoclonal [4D2] to Rhodopsin (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: WB, ICC/IF, IHC-P, IHC-Fr, ELISA, IPmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human, Pig, Bird, Fish, Amphibian
Predicted to work with: MammalsDoes not react with: Drosophila melanogaster, A wide range of other invertebrates
Recombinant fragment corresponding to Bovine Rhodopsin (N terminal).
Database link: P02699
- Rat eye lysate.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab183399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml.
1 μg/mL of ab183399 was sufficient for detection of rhodopsin in 10μg of rat eye lysate by colorimetric immunoblot analysis.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at 4 µg/mg of lysate.|
FunctionPhotoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
Tissue specificityRod shaped photoreceptor cells which mediates vision in dim light.
Involvement in diseaseRetinitis pigmentosa 4
Night blindness, congenital stationary, autosomal dominant 1
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
modificationsPhosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore.
Cellular localizationMembrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
- Information by UniProt
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This product has been referenced in:
- Liu F et al. Pathogenic mutations in retinitis pigmentosa 2 predominantly result in loss of RP2 protein stability in humans and zebrafish. J Biol Chem 292:6225-6239 (2017). Read more (PubMed: 28209709) »
- Liu F et al. Knockout of RP2 decreases GRK1 and rod transducin subunits and leads to photoreceptor degeneration in zebrafish. Hum Mol Genet 24:4648-59 (2015). Read more (PubMed: 26034134) »