Anti-Rhodopsin antibody [4D2] (FITC) (ab183399)
Mouse monoclonal Rhodopsin antibody [4D2] conjugated to FITC. Validated in WB, IP, ELISA, IHC, ICC/IF and tested in Mouse, Rat, Cow, Human, Pig, Bird, Fish, Amphibian. Cited in 2 publication(s).
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-Rhodopsin antibody [4D2] (FITC)
See all Rhodopsin primary antibodies -
Description
Mouse monoclonal [4D2] to Rhodopsin (FITC) -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, IHC-Fr, ELISA, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human, Pig, Bird, Fish, Amphibian
Predicted to work with: MammalsDoes not react with: Drosophila melanogaster, A wide range of other invertebrates
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Immunogen
Recombinant fragment corresponding to Bovine Rhodopsin (N terminal).
Database link: P02699 -
Positive control
- Rat eye lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4D2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab183399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 1 µg/ml. 1 μg/mL of ab183399 was sufficient for detection of rhodopsin in 10μg of rat eye lysate by colorimetric immunoblot analysis. |
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ICC/IF | Use at an assay dependent concentration. | |
IHC-P | Use at an assay dependent concentration. | |
IHC-Fr | Use at an assay dependent concentration. | |
ELISA | Use at an assay dependent concentration. | |
IP | Use at 4 µg/mg of lysate. |
Target
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Function
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal. -
Tissue specificity
Rod shaped photoreceptor cells which mediates vision in dim light. -
Involvement in disease
Retinitis pigmentosa 4
Night blindness, congenital stationary, autosomal dominant 1 -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. -
Post-translational
modificationsPhosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore. -
Cellular localization
Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia. - Information by UniProt
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Database links
- Entrez Gene: 509933 Cow
- Entrez Gene: 100307115 Human
- Entrez Gene: 6010 Human
- Entrez Gene: 212541 Mouse
- Entrez Gene: 397437 Pig
- Entrez Gene: 24717 Rat
- Omim: 180380 Human
- SwissProt: P02699 Cow
see all -
Alternative names
- CSNBAD1 antibody
- MGC138309 antibody
- MGC138311 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [4D2] (FITC) (ab183399)
Immunohistchemical analysis of Mouse retina tissue labeling Rhodopsin. Samples were incubated with primary anitbody at 1:1000 dilution. A FITC Goat anti-mouse (green) was used as the secondary antibody. DAPI (blue) used as a nuclear counterstain.
Protocols
References
This product has been referenced in:
- Liu F et al. Pathogenic mutations in retinitis pigmentosa 2 predominantly result in loss of RP2 protein stability in humans and zebrafish. J Biol Chem 292:6225-6239 (2017). Read more (PubMed: 28209709) »
- Liu F et al. Knockout of RP2 decreases GRK1 and rod transducin subunits and leads to photoreceptor degeneration in zebrafish. Hum Mol Genet 24:4648-59 (2015). Read more (PubMed: 26034134) »