Anti-Rhodopsin antibody [4D2] (FITC) (ab183399)

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Key features and details

  • Mouse monoclonal [4D2] to Rhodopsin (FITC)
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Cow, Human, Pig, Bird, Fish, Amphibian
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

Overview

  • Product name

    Anti-Rhodopsin antibody [4D2] (FITC)
    See all Rhodopsin primary antibodies

  • Description

    Mouse monoclonal [4D2] to Rhodopsin (FITC)

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Pig, Bird, Fish, Amphibian
    Predicted to work with: MammalsDoes not react with: Drosophila melanogaster, A wide range of other invertebrates

  • Immunogen

    Recombinant fragment corresponding to Bovine Rhodopsin (N terminal).
    Database link: P02699

  • Positive control

    • Rat eye lysate.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab183399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.

  • Tissue specificity

    Rod shaped photoreceptor cells which mediates vision in dim light.

  • Involvement in disease

    Retinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

  • Post-translational
    modifications

    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.

  • Cellular localization

    Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [4D2] (FITC) (ab183399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [4D2] (FITC) (ab183399)

    Immunohistchemical analysis of Mouse retina tissue labeling Rhodopsin. Samples were incubated with primary anitbody at 1:1000 dilution. A FITC Goat anti-mouse (green) was used as the secondary antibody. DAPI (blue) used as a nuclear counterstain. 

References (3)

Publishing research using ab183399? Please let us know so that we can cite the reference in this datasheet.

ab183399 has been referenced in 3 publications.

  • Zhao Q  et al. Comparative Transcriptome Profiling of the Loaches Triplophysa bleekeri and Triplophysa rosa Reveals Potential Mechanisms of Eye Degeneration. Front Genet 10:1334 (2019). PubMed: 32010191
  • Liu F  et al. Pathogenic mutations in retinitis pigmentosa 2 predominantly result in loss of RP2 protein stability in humans and zebrafish. J Biol Chem 292:6225-6239 (2017). PubMed: 28209709
  • Liu F  et al. Knockout of RP2 decreases GRK1 and rod transducin subunits and leads to photoreceptor degeneration in zebrafish. Hum Mol Genet 24:4648-59 (2015). PubMed: 26034134

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