Anti-Rhodopsin antibody [4D2] (FITC) (ab183399)

Mouse monoclonal Rhodopsin antibody [4D2] conjugated to FITC. Validated in WB, IP, ELISA, IHC, ICC/IF and tested in Mouse, Rat, Cow, Human, Pig, Bird, Fish, Amphibian. Cited in 2 publication(s).

Submit a review Submit a question References (2)

Overview

  • Product name

    Anti-Rhodopsin antibody [4D2] (FITC)
    See all Rhodopsin primary antibodies

  • Description

    Mouse monoclonal [4D2] to Rhodopsin (FITC)

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, IHC-Fr, ELISA, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Pig, Bird, Fish, Amphibian
    Predicted to work with: MammalsDoes not react with: Drosophila melanogaster, A wide range of other invertebrates

  • Immunogen

    Recombinant fragment corresponding to Bovine Rhodopsin (N terminal).
    Database link: P02699

  • Positive control

    • Rat eye lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab183399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml.

1 μg/mL of ab183399 was sufficient for detection of rhodopsin in 10μg of rat eye lysate by colorimetric immunoblot analysis.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at 4 µg/mg of lysate.

Target

  • Function

    Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.

  • Tissue specificity

    Rod shaped photoreceptor cells which mediates vision in dim light.

  • Involvement in disease

    Retinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

  • Post-translational
    modifications

    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.

  • Cellular localization

    Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [4D2] (FITC) (ab183399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [4D2] (FITC) (ab183399)

    Immunohistchemical analysis of Mouse retina tissue labeling Rhodopsin. Samples were incubated with primary anitbody at 1:1000 dilution. A FITC Goat anti-mouse (green) was used as the secondary antibody. DAPI (blue) used as a nuclear counterstain. 

References

This product has been referenced in:

  • Liu F  et al. Pathogenic mutations in retinitis pigmentosa 2 predominantly result in loss of RP2 protein stability in humans and zebrafish. J Biol Chem 292:6225-6239 (2017). Read more (PubMed: 28209709) »
  • Liu F  et al. Knockout of RP2 decreases GRK1 and rod transducin subunits and leads to photoreceptor degeneration in zebrafish. Hum Mol Genet 24:4648-59 (2015). Read more (PubMed: 26034134) »
See all 2 Publications for this product

Publishing research using ab183399? Please let us know so that we can cite the reference in this datasheet.

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