Product nameAnti-Rhodopsin antibody [4D2] (HRP)
See all Rhodopsin primary antibodies
DescriptionMouse monoclonal [4D2] to Rhodopsin (HRP)
Tested applicationsSuitable for: IP, ICC/IF, IHC-P, IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human, Xenopus laevis, Bird, Fish, Amphibian
Predicted to work with: MammalsDoes not react with: Drosophila melanogaster, A wide range of other invertebrates
Other Immunogen Type corresponding to Bovine Rhodopsin (N terminal).
Database link: P02699
- Mouse retina tissue and rat eye lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
- Anti-Rhodopsin antibody [Rho 4D2] (ab98887)
Our Abpromise guarantee covers the use of ab183400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa.
1 μg/mL of ab183400 was sufficient for detection of rhodopsin in 10μg of rat eye lysate by colorimetric immunoblot analysis
FunctionPhotoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
Tissue specificityRod shaped photoreceptor cells which mediates vision in dim light.
Involvement in diseaseRetinitis pigmentosa 4
Night blindness, congenital stationary, autosomal dominant 1
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
modificationsPhosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore.
Cellular localizationMembrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
- Information by UniProt
- CSNBAD1 antibody
- MGC138309 antibody
- MGC138311 antibody
ab183400 has not yet been referenced specifically in any publications.