Anti-Rhodopsin antibody [A531] (ab190307)
Key features and details
- Mouse monoclonal [A531] to Rhodopsin
- Suitable for: ICC/IF, WB
- Reacts with: Cow, Pig
- Isotype: IgG1
Overview
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Product name
Anti-Rhodopsin antibody [A531]
See all Rhodopsin primary antibodies -
Description
Mouse monoclonal [A531] to Rhodopsin -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Cow, Pig
Predicted to work with: Sheep, Rabbit, Cat, Dog, Cynomolgus monkey, Chinese hamster -
Immunogen
Full length native protein (purified) corresponding to Cow Rhodopsin aa 1 to the C-terminus.
Database link: P02699 -
Positive control
- Bovine retinal extracts; Pig retinal tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
A531 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000.
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WB |
1/5000. Use under non reducing condition. Detects a band of approximately 35,70,140 kDa (predicted molecular weight: 39 kDa).
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Notes |
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ICC/IF
1/1000. |
WB
1/5000. Use under non reducing condition. Detects a band of approximately 35,70,140 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal. -
Tissue specificity
Rod shaped photoreceptor cells which mediates vision in dim light. -
Involvement in disease
Retinitis pigmentosa 4
Night blindness, congenital stationary, autosomal dominant 1 -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. -
Post-translational
modificationsPhosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore. -
Cellular localization
Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia. - Information by UniProt
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Database links
- Entrez Gene: 493762 Cat
- Entrez Gene: 509933 Cow
- Entrez Gene: 493763 Dog
- Entrez Gene: 397437 Pig
- Entrez Gene: 100009200 Rabbit
- SwissProt: Q95KU1 Cat
- SwissProt: P28681 Chinese hamster
- SwissProt: P02699 Cow
see all -
Alternative names
- CSNBAD1 antibody
- MGC138309 antibody
- MGC138311 antibody
see all
Images
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Immunofluorescent analysis of pig retinal tissue labeling Rhodopsin with ab190307 at 1/1000 dilution (green) and counterstained with rabbit polyclonal antibody to neurofilament (red) and DNA (blue).
Rhodopsin is most abundant in the outer segments of retina (OS), NF-M is abundant in the optic nerve fiber layer (ONFL), but seen in processes and neurons in other regions also. Other layers are pigmented epithelium (PE), outer and inner nuclear layers (ONL, INL), outer and inner plexiform layers (OPL, IPL) and ganglion cell layer (GCL).
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Anti-Rhodopsin antibody [A531] (ab190307) at 1/5000 dilution + Bovine retinal extract
Predicted band size: 39 kDaBands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin.
Note, due to the highly hydrophobic nature of rhodopsin, it is important not to boil a sample containing it in SDS-PAGE sample buffer, as this will result in more extensive aggregation of the Rhodopsin protein.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab190307 has been referenced in 3 publications.
- Urbano-Gámez JD et al. Biocompatibility Study of a Commercial Printed Circuit Board for Biomedical Applications: Lab-on-PCB for Organotypic Retina Cultures. Micromachines (Basel) 12:N/A (2021). PubMed: 34945319
- Valdés-Sánchez L et al. Retinal pigment epithelium degeneration caused by aggregation of PRPF31 and the role of HSP70 family of proteins. Mol Med 26:1 (2019). PubMed: 31892304
- Cehofski LJ et al. IL-18 and S100A12 Are Upregulated in Experimental Central Retinal Vein Occlusion. Int J Mol Sci 19:N/A (2018). PubMed: 30366444