Key features and details
- Mouse monoclonal [A531] to Rhodopsin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Cow, Human, Pig
- Isotype: IgG1
Product nameAnti-Rhodopsin antibody [A531]
See all Rhodopsin primary antibodies
DescriptionMouse monoclonal [A531] to Rhodopsin
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human, Pig
Predicted to work with: Sheep, Rabbit, Cat, Dog, Cynomolgus monkey, Chinese hamster
Full length native protein (purified) corresponding to Cow Rhodopsin aa 1-348.
MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIML GFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLH GYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGE NHAIMGVAFTWVMALACAAPPLVGWSRYIPEGMQCSCGIDYYTPHEETNN ESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEV TRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAV YNPVIYIMMNKQFRNCMVTTLCCGKNPLGDDEASTTVSKTETSQVAPA
Database link: P02699
- Bovine retinal extracts; Pig retinal tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab190307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Use under non reducing condition. Detects a band of approximately 35,70,140 kDa (predicted molecular weight: 39 kDa).|
FunctionPhotoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
Tissue specificityRod shaped photoreceptor cells which mediates vision in dim light.
Involvement in diseaseRetinitis pigmentosa 4
Night blindness, congenital stationary, autosomal dominant 1
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
modificationsPhosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore.
Cellular localizationMembrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
- Information by UniProt
- CSNBAD1 antibody
- MGC138309 antibody
- MGC138311 antibody
Immunofluorescent analysis of pig retinal tissue labeling Rhodopsin with ab190307 at 1/1000 dilution (green) and counterstained with rabbit polyclonal antibody to neurofilament (red) and DNA (blue).
Rhodopsin is most abundant in the outer segments of retina (OS), NF-M is abundant in the optic nerve fiber layer (ONFL), but seen in processes and neurons in other regions also. Other layers are pigmented epithelium (PE), outer and inner nuclear layers (ONL, INL), outer and inner plexiform layers (OPL, IPL) and ganglion cell layer (GCL).
Anti-Rhodopsin antibody [A531] (ab190307) at 1/5000 dilution + Bovine retinal extract
Predicted band size: 39 kDa
Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin.
Note, due to the highly hydrophobic nature of rhodopsin, it is important not to boil a sample containing it in SDS-PAGE sample buffer, as this will result in more extensive aggregation of the Rhodopsin protein.
ab190307 has been referenced in 2 publications.
- Valdés-Sánchez L et al. Retinal pigment epithelium degeneration caused by aggregation of PRPF31 and the role of HSP70 family of proteins. Mol Med 26:1 (2019). PubMed: 31892304
- Cehofski LJ et al. IL-18 and S100A12 Are Upregulated in Experimental Central Retinal Vein Occlusion. Int J Mol Sci 19:N/A (2018). PubMed: 30366444