Key features and details
- Rabbit polyclonal to Rhodopsin
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Rhodopsin antibody
See all Rhodopsin primary antibodies
DescriptionRabbit polyclonal to Rhodopsin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Rabbit, Pig
Synthetic peptide corresponding to Human Rhodopsin aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- WB: Y79 and WERI whole cell lysates. IHC-P: Rat normal eye tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab112576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 39 kDa).|
|IHC-P||Use a concentration of 0.5 µg/ml.
The optimal concentration will need to be determined. 0.5ug/mL gave a good result with a polymer-HRP secondary antibody reagent.
FunctionPhotoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
Tissue specificityRod shaped photoreceptor cells which mediates vision in dim light.
Involvement in diseaseRetinitis pigmentosa 4
Night blindness, congenital stationary, autosomal dominant 1
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
modificationsPhosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore.
Cellular localizationMembrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
- Information by UniProt
- CSNBAD1 antibody
- MGC138309 antibody
- MGC138311 antibody
IHC image of Rhodopsin staining in Rat normal eye formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112576, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-Rhodopsin antibody (ab112576) at 1 µg/ml
Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 2 : WERI (Human Retinoblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
The predicted molecular weight of Rhodopsin is 39 kDa (SwissProt), however we expect to observe a banding pattern around 48 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab112576 has been referenced in 2 publications.
- Mitra RN et al. Genomic form of rhodopsin DNA nanoparticles rescued autosomal dominant Retinitis pigmentosa in the P23H knock-in mouse model. Biomaterials 157:26-39 (2018). PubMed: 29232624
- Eleftheriou CG et al. Meclofenamic acid improves the signal to noise ratio for visual responses produced by ectopic expression of human rod opsin. Mol Vis 23:334-345 (2017). Histology ; Mouse . PubMed: 28659709