Overview

  • Product name

    Anti-Rhodopsin antibody [B630]
    See all Rhodopsin primary antibodies
  • Description

    Mouse monoclonal [B630] to Rhodopsin
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Cow, Human, Pig
    Predicted to work with: Mouse, Rat
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • IHC-P: Human and pig retina tissue. WB: Bovine retinal extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.07% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Protein G purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    B630
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/5000.

Note: Due to the highly hydrophobic nature of rhodopsin, it's important to avoid boiling samples containing this protein in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the rhodopsin protein and appearance of more of this high molecular weight material.

ICC/IF 1/1000.

Target

  • Function

    Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
  • Tissue specificity

    Rod shaped photoreceptor cells which mediates vision in dim light.
  • Involvement in disease

    Retinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • Post-translational
    modifications

    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.
  • Cellular localization

    Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Information by UniProt
  • Database links

  • Alternative names

    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human retinal tissue stained for Rhodopsin with ab230692 at 10 µg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded pig retinal tissue stained for Rhodopsin with ab230692 (green) at 10 µg/ml in immunohistochemical analysis.

    Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).

  • Anti-Rhodopsin antibody [B630] (ab230692) at 1/5000 dilution + bovine retinal extracts

    Developed using the ECL technique.


    Bands about 70 kDa and 140 kDa are aggregated forms of rhodopsin.

References

ab230692 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab230692.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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