Key features and details
- Mouse monoclonal [B630] to Rhodopsin
- Suitable for: IHC-P, WB
- Reacts with: Cow, Human, Pig
- Isotype: IgG1
Product nameAnti-Rhodopsin antibody [B630]
See all Rhodopsin primary antibodies
DescriptionMouse monoclonal [B630] to Rhodopsin
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Cow, Human, Pig
Predicted to work with: Mouse, Rat
The details of the immunogen for this antibody are not available.
- IHC-P: Human and pig retina tissue. WB: Bovine retinal extracts.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.07% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesProtein G purified from tissue culture supernatant.
Light chain typekappa
Our Abpromise guarantee covers the use of ab230692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Note: Due to the highly hydrophobic nature of rhodopsin, it's important to avoid boiling samples containing this protein in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the rhodopsin protein and appearance of more of this high molecular weight material.
FunctionPhotoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
Tissue specificityRod shaped photoreceptor cells which mediates vision in dim light.
Involvement in diseaseRetinitis pigmentosa 4
Night blindness, congenital stationary, autosomal dominant 1
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
modificationsPhosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore.
Cellular localizationMembrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
- Information by UniProt
- CSNBAD1 antibody
- MGC138309 antibody
- MGC138311 antibody
Formalin-fixed, paraffin-embedded human retinal tissue stained for Rhodopsin with ab230692 at 10 µg/ml in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded pig retinal tissue stained for Rhodopsin with ab230692 (green) at 10 µg/ml in immunohistochemical analysis.
Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).
Anti-Rhodopsin antibody [B630] (ab230692) at 1/5000 dilution + bovine retinal extracts
Developed using the ECL technique.
Bands about 70 kDa and 140 kDa are aggregated forms of rhodopsin.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230692 has not yet been referenced specifically in any publications.