RecombinantRabMAb

Recombinant Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)

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Western blot - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
  • Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7996] to Rhodopsin - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free
    See all Rhodopsin primary antibodies

  • Description

    Rabbit monoclonal [EPR7996] to Rhodopsin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human Rhodopsin aa 300-400. The exact sequence is proprietary.

  • General notes

    ab249189 is the carrier-free version of ab155097 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab249189 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab249189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Function

      Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.

    • Tissue specificity

      Rod shaped photoreceptor cells which mediates vision in dim light.

    • Involvement in disease

      Retinitis pigmentosa 4
      Night blindness, congenital stationary, autosomal dominant 1

    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

    • Post-translational
      modifications

      Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
      Contains one covalently linked retinal chromophore.

    • Cellular localization

      Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
    • Information by UniProt

    • Database links

      • Alternative names

        • CSNBAD1 antibody
        • MGC138309 antibody
        • MGC138311 antibody
        • OPN 2 antibody
        • OPN2 antibody
        • opsd antibody
        • OPSD_HUMAN antibody
        • opsin 2 antibody
        • Opsin 2 rod pigment antibody
        • Opsin-2 antibody
        • Opsin2 antibody
        • Retinitis Pigmentosa 4 antibody
        • Retinitis pigmentosa 4 autosomal dominant antibody
        • RHO antibody
        • Rhodopsin antibody
        • RP 4 antibody
        • RP4 antibody
        see all

      Images

      • Western blot - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
        Western blot - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
        Anti-Rhodopsin antibody [EPR7996] (ab155097) at 1/1000 dilution + Rat eyeball lysate at 10 µg

        Secondary
        Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 39 kDa



        This data was developed using ab155097, the same antibody clone in a different buffer formulation.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
        This data was developed using ab155097, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling Rhodopsin with ab155097 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
        This data was developed using ab155097, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab155097 showing -ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
        This data was developed using ab155097, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal pancreas tissue using ab155097 showing -ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
        This data was developed using ab155097, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue using ab155097 showing -ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
        This data was developed using ab155097, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155097 showing -ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
        Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab249189? Please let us know so that we can cite the reference in this datasheet.

      ab249189 has not yet been referenced specifically in any publications.

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