Recombinant Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free
See all Rhodopsin primary antibodies -
Description
Rabbit monoclonal [EPR7996] to Rhodopsin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human Rhodopsin aa 300-400. The exact sequence is proprietary.
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General notes
ab249189 is the carrier-free version of ab155097 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab249189 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7996 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab249189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 39 kDa. |
Target
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Function
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal. -
Tissue specificity
Rod shaped photoreceptor cells which mediates vision in dim light. -
Involvement in disease
Retinitis pigmentosa 4
Night blindness, congenital stationary, autosomal dominant 1 -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. -
Post-translational
modificationsPhosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore. -
Cellular localization
Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia. - Information by UniProt
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Database links
- Entrez Gene: 100307115 Human
- Entrez Gene: 6010 Human
- Entrez Gene: 24717 Rat
- Omim: 180380 Human
- SwissProt: P08100 Human
- SwissProt: P51489 Rat
- Unigene: 247565 Human
- Unigene: 92530 Rat
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Alternative names
- CSNBAD1 antibody
- MGC138309 antibody
- MGC138311 antibody
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Datasheets and documents
References
ab249189 has not yet been referenced specifically in any publications.
