Recombinant Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free (ab249189)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7996] to Rhodopsin - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Rhodopsin antibody [EPR7996] - BSA and Azide free
See all Rhodopsin primary antibodies -
Description
Rabbit monoclonal [EPR7996] to Rhodopsin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249189 is the carrier-free version of ab155097.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7996 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa. |
Target
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Function
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal. -
Tissue specificity
Rod shaped photoreceptor cells which mediates vision in dim light. -
Involvement in disease
Retinitis pigmentosa 4
Night blindness, congenital stationary, autosomal dominant 1 -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. -
Post-translational
modificationsPhosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore. -
Cellular localization
Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia. - Information by UniProt
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Database links
- Entrez Gene: 100307115 Human
- Entrez Gene: 6010 Human
- Entrez Gene: 24717 Rat
- Omim: 180380 Human
- SwissProt: P08100 Human
- SwissProt: P51489 Rat
- Unigene: 247565 Human
- Unigene: 92530 Rat
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Alternative names
- CSNBAD1 antibody
- MGC138309 antibody
- MGC138311 antibody
see all
Images
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This data was developed using ab155097, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling Rhodopsin with ab155097 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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This data was developed using ab155097, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab155097 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
This data was developed using ab155097, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue using ab155097 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249189 has not yet been referenced specifically in any publications.