Overview

  • Product name

    Anti-Rhodopsin antibody [Rho 4D2]
    See all Rhodopsin primary antibodies
  • Description

    Mouse monoclonal [Rho 4D2] to Rhodopsin
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ELISA, IHC-P, IHC-Fr, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Pig, Xenopus laevis
    Does not react with: Drosophila melanogaster
  • Immunogen

    Recombinant fragment corresponding to Bovine Rhodopsin (N terminal).
    Database link: P02699

Properties

Applications

Our Abpromise guarantee covers the use of ab98887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB 1/1000. Predicted molecular weight: 39 kDa.
IP Use at an assay dependent concentration. 1-2 µg per 100-500µg of protein.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • Function

    Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
  • Tissue specificity

    Rod shaped photoreceptor cells which mediates vision in dim light.
  • Involvement in disease

    Retinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • Post-translational
    modifications

    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.
  • Cellular localization

    Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Information by UniProt
  • Database links

  • Alternative names

    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

Images

  • Immunohistochemistry analysis staining Rhodopsin in mouse retina with ab98887 at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain.

  • Overlay histogram showing Y79 cells stained with ab98887 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab98887, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Y79 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:

  • Daruich A  et al. Iron is neurotoxic in retinal detachment and transferrin confers neuroprotection. Sci Adv 5:eaau9940 (2019). Read more (PubMed: 30662950) »
  • Zou T  et al. Organoid-derived C-Kit+/SSEA4- human retinal progenitor cells promote a protective retinal microenvironment during transplantation in rodents. Nat Commun 10:1205 (2019). Read more (PubMed: 30872578) »
See all 13 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Western blot
Sample
Bird Tissue lysate - whole (Pigeon Retina, no boiling of sample prior to loadi)
Gel Running Conditions
Non-reduced Denaturing (7.5% Tris-Glycine)
Loading amount
20 µg
Specification
Pigeon Retina, no boiling of sample prior to loadi
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Tobias Hochstöger

Verified customer

Submitted Sep 15 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (12% Gel)
Loading amount
2 µg
Specification
Retina
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Onkar Sawant

Verified customer

Submitted Mar 04 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Eye)
Antigen retrieval step
None
Permeabilization
Yes - Tween 0.1%
Specification
Eye
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2 · Temperature: 24°C
Fixative
Paraformaldehyde

Onkar Sawant

Verified customer

Submitted Jan 28 2016

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