Anti-RhoF antibody [1D4B6] (ab201976)
Key features and details
- Mouse monoclonal [1D4B6] to RhoF
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-RhoF antibody [1D4B6]
See all RhoF primary antibodies -
Description
Mouse monoclonal [1D4B6] to RhoF -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment corresponding to Human RhoF aa 1-100 (N terminal).
Database link: Q9HBH0 -
Positive control
- Recombinant Human RhoF protein, RohF transfected HEK293 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1D4B6 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 24 kDa.
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Flow Cyt |
1/200 - 1/400.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 24 kDa. |
Flow Cyt
1/200 - 1/400. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 54509 Human
- Entrez Gene: 23912 Mouse
- SwissProt: Q9HBH0 Human
- SwissProt: Q8BYP3 Mouse
- Unigene: 644504 Human
- Unigene: 707579 Human
- Unigene: 720066 Human
- Unigene: 253876 Mouse
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Alternative names
- ARHF antibody
- FLJ20247 antibody
- Ras homolog gene family member F (in filopodia) antibody
see all
Images
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Anti-RhoF antibody [1D4B6] (ab201976) at 1/500 dilution + RhoF recombinant protein
Predicted band size: 24 kDa -
All lanes : Anti-RhoF antibody [1D4B6] (ab201976) at 1/500 dilution
Lane 1 : Untransfected HEK293 cell lysate
Lane 2 : RhoF transfected HEK293 cell lysate
Predicted band size: 24 kDa -
Flow cytometric analysis of Hela cells labeling RhoF using ab201976 at 1/200 dilution (green) and negative control (red).
Datasheets and documents
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Datasheet download
References (0)
ab201976 has not yet been referenced specifically in any publications.